Imaging Core Facility

Imaging Core Facility

 The Imaging Facility gives researchers access to sophisticated fluorescence microscopes for the observation and analysis of live or fixed organisms and cells. The scale of samples ranges from whole (small) organisms to single molecules with super-resolution microscopy. We also have high-end flow cytometers, which allow analysis and sorting of cells in many fluorescence channels at high speed. Users get personal training to use equipment and software as needed for their specific experiments, as well as support for data processing and especially quantitative image analysis.

Microscopy

Currently we have five light microscopes:

  • WF1: Widefield microscope GE DeltaVision Elite, including laser module for TIRF and photo bleaching /switching)
  • WF2: Widefield microscope Leica Thunder with high-sensitivity sCMOS camera, image splitter and lasers for TIRF and fast scanning for photo switching / bleaching
  • CF1: Laser-scanning confocal microscope Zeiss LSM780, including a module for FCS and RICS
  • CF2: Laser-scanning confocal microscope Leica SP8 FALCON, including White Light Laser and fully integrated FLIM module
  • SR1: Super-resolution microscope Zeiss Elyra PS.1 with modules for SMLM (PALM, STORM, PAINT) and SIM, as well as dual-camera module with image splitter

All systems are equipped with motorised stage, temperature control and CO2 incubation.

Flow Cytometry

For the analysis of cells we offer access to a Thermofisher Attune NxT high-speed flow cytometer with automated plate reader, for fluorescence-activated cell sorting (FACS) we have a BD FACSAria III. Both are equipped with excitation lasers ranging from UV (375 and 405 nm) to red (633 nm), and emission filters for practically all fluorophores.

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