Publications
bioRxiv
Combinatorial selective ER-phagy remodels the ER during neurogenesis
Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, Harper JW
bioRixv 2023 Nov 10:2023.06.26.546565. doi: 10.1101/2023.0626.546565. preprint
Insights into the ISG 15 transfer cascade by the UBE 1L activating enzyme
Wallace I, Baek K, Prabu JR, Vollrath R, von Gronau S, Schulman BA, Swatek KN
Nat Commun. 2023 Dec 2;14(1); 7970. doi: https://10.1038/s41467-023-43711-3
K6-linked ubiquitylation marks formaldehyde-induced RNA-protein crosslinks for resolution
Suryo Rahmanto AS, Blum CJ, Scalera C, Heidelberger JB, Mesitov M, Horn-Ghetko D, Gräf JF, Mikicic I, Hobrecht R, Orekhova A, Ostermaier M, Ebersberger S, Möckel MM; Krapoth N, Da Silva Fernandes N, Mizi A, Zhu Y, Chen JX, Choudhary C, Papantonis A, Ulrich HD, Schulman BA, König J, Beli P
Mol Cell. 2023 Nov 7;83: 1-18 doi: 10.1016/j.molcel.202310.011 online ahead of print
Catalysis of non-canonical protein ubiquitylation by the ARIH1 ubiquitin ligase
Purser B, Tripathi-Giesgen I, Li J, Scott DC, Horn-Ghetko D, Baek K, Schulman BA, Alpi A, Kleiger G
Biochem J. 2023 Nov 29;480(22): 1817-1831 doi: 10.1042/BCJ20230373
Activity-based profiling cullin-RING ligase networks by conformation-specific probes
Henneberg L, Singh J, Duda DM, Baek K, Yanishevski D, Murray PJ, Mann M, Sidhu SS, Schulman BA
Nat Chem Biol. 2023 Dec 19(12): 1513-1523 doi: 10.1038/s41589-023-01392-5 Epub 2023 Aug 31
Structural snapshots along K48-linked ubiquitin chain formation by the HECT E3 UBR5
Hehl LA, Horn-Ghetko D, Prabu JR, Vollrath R, Tung Vu D, Perrez Berrocal DA, Mulder MPC, van der Heden van Noort GJ, Schulman BA
Nat Chem Biol. 2023 Aug 24 doi: 10.1038/s41589-023-01414-2 online ahead of print
A Pro-fluorescent Ubiquitin based probe to monitor cysteine-based E3 ligase activity
Perez Berrocal DA, Vishwanatha TM, Horn-Ghetko D, Botsch JJ, Hehl LA, KostrhonS, Misra M, Dikic I, Geurink PP, van Dam H, Schulman BA, Mulder MPC
Angew Chem Int Ed Eng 2023 Aug 7;63(32): e202303319 doi: 10.1002/anie.202303319 Epub 2023 Jun 27
To be (in a transcriptional complex) or not to be (promoting UBR5 ubiquitylation): That is an answer to how degradation controls gene expression
Hehl LA, Schulman BA,
Mol Cell. 2023 Aug 3;83(15):2616-2618 doi: 10.1016/j.molcell.2023.07.010
PARK15/FBXO7 is dispensable for PINKI/Parkin mitophagy in iNeurons and HeLa cell systems
Kraus F, Goodall EA, Smith ER, Jiang Y, Paoli JC, Adolf F, Paulo JA, Schulman BA, Harper JW
EMBO Rep. 2023 Aug 3;24(8): e56399 doi: https://10.15252/embr.202256399 Epub 2023 Jun 19
Ubiquitination regulates ER-phagy and remodelling of endoplasmatic reticulum
Gonzalez A, Covarrubias-Pinto A, Bhaskara RM, Glogger M, Kuncha SK, Xavier A, Seemann E, Misra M, Hoffmann ME, Bräuning B, Balakrishnan A, Qualmann B, Dötsch V, Schulman BA, Kessels MM, Hübner CA, Heilemann M, Hummer G, Dikic I.
Nature 2023 Jun 618(7964):394-401 doi: 10.1038/s41586-023-06089-2 Epub 2023 May 24
Systemwide disassembly and assembly of SCF ubiquitin ligase complexes
Baek K, Scott DC, Henneberg LT, King MT, Mann M, Schulman BA,
Cell 2023 Apr 27; 186( 9):1895-1911, doi: 10.1016 /j.cell.2023.02.035. Epub 2023 Apr 6
An expanded lexicon for the ubiquitin code
Dikic I and Schulman BA,
Nat Rev Mol Cell Biol 2023 Apr 24(4) ;273-287 doi: 10.1038/s41580-022-00543-1. Epub 2022 Oct 25
In situ snapshots along a mammalian selective autophagy pathway
Li M, Tripathi-Giesgen I, Schulman BA, Baumeister W, Wilfling F
Proc Natl Acad Sci USA. 2023 Mar21;120(12):e2221712120. doi: 10.1073/pnas22211712120. Epup 2023 Mar 14
E3 ligase autoinhibition by C-degron mimicry maintains C-degron substrate fidelity
Scott DC, King MT, Baek K, Gee CT, Kalathur R, Li J, Purset N, Nourse A, Chai SC, Vaithiyalingam S, Chen T, Lee RE, Elledge SJ, Kleiger G, Schulman BA
Mol Cell. 2023 Feb 16: doi: 10.1016/j.molcel.2023.01.019
A central role for regulated protein stability in the control of TFE3 and MITF by nutrients
Nardone C, Palanski BA, Scott DC, Timms RT, Barber KW, Gu X, Mao A, Leng Y, Watson EV, Schulman BA, Cole PA, Elledge SJ
Mol Cell. 2023 Jan 5;83:57-73 doi: 10.1016/j.molcel.2022.12.013
Modular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation
Sherpa D, Müller J, Karayel O, Chrustowicz J, Xu P, Gottemukkala, KV, Baumann C, Gross A, Czarneki O, Zhang W, Gu J, Nilvebrant J, Weiss MJ, Sidhu SS, Murray PJ, Mann M, Schulman BA, Alpi A
elife. 2022 Dec 2;11:e77937. doi: 10.7554/eLife.77937
In situ structural analysis reveals membrane shape transitions during autophagosome formation
Bieber A, Capitanio C, Erdmann PS, Fiedler F, Beck F, Lee CW, Li D, Hummer G, Schulman BA, Baumeister W, Wilfling F
Proc Natl Acad Sci USA 2022 Sep 27;119(39):2209823119 doi: 10.1073/pnas.2209823119. Epub 2022 Sep 19
Structure of CRL7FBXW8 reveals coupling with CUL1-RBX1/ROC1 for multi-cullin-RING E3 catalyzed ubiquitin ligation
Hopf L, Baek K, Klügel M, von Gronau S, Xiong Y, Schulman BA
Nat Struct Mol Biol 2022 Aug 18; doi: 10.1038/s41594-022-00815-6
Cryo-EM structures of Gid12-bound GID E3 reveal steric blockade as a mechanism inhibiting substarte ubiquitylation
Qiao S, Lee CW, Sherpa D, Chrustowicz J, Cheng J, Duennebacke M, Steigenberger B,, Karayel O, Vu DT, von Gronau S, Mann M, Wilfling F, Schulman BA
Nat Commun. 2022 Jun 1;13(1):3041; doi: 10.1038/s41467-022-30803-9
The structural context of PTMs at a proteome wide scale
Bludau I, Willems S, Zeng WF, Strauss MT, Hansen FM Tanzer MC, Karayel O, Schulman BA, Mann M
PLOS Biol. 2022 May 16;20(5):e3001636; doi: 10.1371/journal.pbio.3001636. ecollection 2022 May
GID E3 ligase assembly ubiquitinates an RSP5 E3 adaptor and regulates plasma membrane transporters
Langlois CR, Beier V, Karayel O, Chrustowicz J, Sherpa D, Mann M, Schulman BA,
EMBO Rep 2022 Apr 19; doi: 10.15252/embr.202153835 (online ahead of print)
How the ends signal the end: Regulation by E3 ubiquitin ligases recognizing protein termini
Sherpa D, Chrustowicz J, Schulman BA,
Mol Cell. 2022 Feb 18:s1097-2765(22)0109-5 doi: 10.1016/j.molcel.2022.02.004. Epub 2022 Mar 4
New classes of E3 ligases illuminated by chemical probes
Horn-Ghetko D, Schulman BA,
Curr Opin Struct Biol. 2022 Feb 24;73:102341 doi: 10.1016/j.sbi.2022.102341. Epub 2022 Feb 25
Targeted protein degradation: from small molecules to complex organelles - a Keystone Symposia report
Cable J, Weber-Ban E, Clausen T, Walters KJ, Sharon M, Finley DJ, Gu Y, Hanna J, Feng Y, Martens S, Hansen M, Zhang H, Goodwin JM, Reggio A, Chang C, Ge L, , Schulman BA, Deshaies RJ, Dikic I, Harper JW, Wertz IE, Thomä NH, Stabicki M, Frydman J, Jakob U, David DC, Bennet EJ, Bertozzi CR, Sardana R, Eapen VV, Carra S.
Ann N Y Acad Sci 2022. Jan 8; doi: 10.1111/nyas.14745. Epub 2022 Jan 8
Multifaceted N-degron recognition and ubiquitylation by GID/CTLH E3 Ligases
Chrustowicz J, Sherpa D, Teyra J, Loke MS, Popowicz GM, Basquin J, Sattler M, Prabu JR, Sidhu SS, Schulman BA,
J Mol Biol. 2022 Jan 30;434 (2):167347 doi: 10.1016/j.jmb.2021.167347. Epub 2021 Nov 9
APC7 mediates ubiquitin signaling in constitutive heterochromatin in the developing mammalian brain
Ferguson CJ, Urso O, Bodrug T, Gassaway BM, Watson ER, Prabu JR, Lara-Gonzalez P, Martinez-Jacin RC, Wu DY, Brigatti KW, Puffenberger EG, Taylor CM, Haas-Givler B, Jinks RN, Strauss KA, Desai A, Gabel HW, Gygi SP, Schulman BA, Brown NG, Bonni A.
Mol Cell.2022 Jan 6;82(1):90-105.e13 doi: 10.1016/j.molcel.2021.11.031. Epub 2021 Dec 22
CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific NEDD8 activation
Kostrhon S, Prabu JR, Baek K, Horn-Ghetko D, von Gronau S, Klügel M, Basquin J, Alpi AF, Schulman BA,
Nat Chem Biol. 2021 Oct;17(10): 1075-1083; doi: 10.1038/s41589-021-00858-8. Epub 2021 Sep 13
Host ubiquitin protein tags lipid to fight bacteria
Schulman BA, Harper JW
Nature. 2021 Jun;594(7861):28-29 doi: 10.1038/d41586-021-01267-6
NEDD8 and ubiquitin ligation by cullin-RING E3 ligases
Baek K, Scott CD, Schulman BA
Curr Opin Struct Biol. 2021 Apr;(67):101-109 doi: 10.1016/j.sbi.2020.10.007. Epub 2020 Nov 5
Improvement of oral bioavailability of Pyrazolo Pyridone inhibitors of the interaction of DCN1/2 and UBE2M
Kim HS, Hammil JT, Scott CD, Chen Y, Rice AL, Pistel W, Singh B, Schulman BA, Guy RK
J Med Chem. 2021 Mai 13;64(9):5850-5862 doi: 10.1021/acs.jmedchem.1c00035. Epub 2021 May 4
GID E3 ligase supramolecular chelate aasembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme
Sherpa D, Chrustowicz J, Qiao S, Langlois CR, Hehl LA, Gottemukkala KV, Hansen FM, Karayel O, von Gronau S, Prabu JR, Mann M, Alpi AF, Schulman BA.
Mol Cell. 2021 Jun 3;81(11):2445-2459 doi: 10.1016/j.molcell.2021.03.025 (Previewed in Mol Cell 81:2270-2272)
Decoding the messaging of the ubiquitin system using chemical and protein probes
Henneberg L, Schulman BA
Cell Chem Biol. 2021 Jul 15;28(7):889-902 doi: 10.1016/j.chembiol.2021.03.009. Epub 2021 apr 7
Cullin RING ubiquitin ligase regulatory circuits: a quartercentury beyond the F-box hypthesis
Harper W, Schulman BA
Annu Rev Biochem. 2021 Jun 20;90:403-429 doi: 10.1146/annurev-biochem-090120-013613. Epub 2021 Apr 6
Linkage-specific ubiquitin chain formation depends on a Lysine hydrocarbon ruler
Liwocha J, Krist DT, van der Heden van Nort GJ, Hansen FM, Truong VH, Karayel O, Purser N, Houston D, Burton N Bostock MJ, Sattler M, Mann M, Harrison JS, Kleiger G, Ovaa H, Schulman BA
Nat Chem Biol. 2021 Mar 17;3:272-279 doi: 10.1038/s41589-020-00696-0. Epub 2020 Dec 7
The UBA domain of conjugating enzyme Ubc1/Ube2K facilitates assembly of K48/K63-branched ubiquitin chains
Pluska L, Jarosch E, Zauber H, Kniss A, Waltho A, Bagola K, von Delbrück M, Löhr F, Schulman BA, Selbach M, Dötsch V, Sommer T.
EMBO J. 2021 Mar 15;40(6):e106094 doi: 10.15252/embj.2020106094. Epub 2021Feb 12
The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma
Heider M, Eichner R, Stroh J, Morath V, Kuisl A, Zecha J, Lawatscheck J, Baek K, Garz AK, Rudelius M, Deuschle FC, Keller U, Lemeer S, Verbeek M, Götze KS, Skerra A, Weber WA, Buchner J, Schulman BA, Kuster B, Fernandez-Saiz V, Bassermann F.
Mol Cell. 2021 Mar 18; 81(6):1170-1186; doi: 10.1016/molcell2020.12.046. Epub 2021 Feb 10
Ubiquitin ligation to F-box protein targets by SCF-RBRE3-E3 super-assembly
Horn-Ghetko D, Krist DT, Prabu RJ, Baek K, Mulder MPC, Klügel M, Scott DC, Ovaa H, Kleiger G, Schulman BA
Nature. 2021 Feb;590(7847):671-676; doi: 10.1038/s41586-021-03197-9. Epub 2021 Feb 3
Data-independant acquisition method for ubiquitinome analysis reveals regulation of circadian biology.
Hansen FM, Tanzer MC, Brüning F, Bludau I, Schulman BA, Robles MS, Karayel O, Mann M.
Nat Commun. 2021 Jan 11;12(1):254 doi: 10.1038/41467-020-20509-1
FBX011-mediatedproteolysis of BAHD1 relieves PRC2 dependent transcriptional repression in erythropoesis
Xu P, Scott DC, Xu B, Yao Y, Feng R, Cheng L, Mayberry K, Bi W, Palmer LE, King MT, Wang H, Li Y, Fan Y, Alpi AF, Li C, Peng J, Papizan J, Pruett-Miller SM, Spallek R, Bassermann F, Cheng Y, Schulman BA, Weiss MJ.
Blood. 2021 Jan 14;137(2):155-167. doi: 10.1182/blood.2020007809
2020
DIA-based systems biology approach unveils novel E3-dependent responses to a metabolic shift.
Karayel O, Michaelis AC, Mann M, Schulman BA, Langlois CR.
Proc Natl Acad Sci USA. 2020 Dec 22;117(51):32806-32815. doi: 10.1073/pnas.2020197117 Epub 2020 Dec 7
A selective autophagy pathway for phase separated endocytic protein deposits.
Wilfling F, Lee C-W, Erdmaann P, Zheng Y, Sherpa D, Jentsch S, Pfander B, Schulman BA, Baumeister W.
Mol Cell. 2020 Dec3 80(5):764-778 doi: 10.1016/j.molcel.2020.10.030 (Previewed Mol Cell 80: 758-759)
Integrative proteomics reveals principles of dynamic phospho-signaling networks in human erythropoiesis.
Karayel O, Xu P, Bludau I, Bhoopalan SV, Yao Y, Colaco ARF, Delgado AS, Schulman BA, Alpi AF, Weiss MJ, Mann M.
Mol Syst Biol. 2020 Dec;16(12)e9813 doi:10.15252/msb.20209813
Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.
Miller-Vedam LE, Bräuning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Craik JS, Schulman BA, Frost A, Weissman JS.
Elife 2020 Nov 25;9:e62611. doi: 10.7554/elife.62611
Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity.
Shin D, Mukherjee R, Grewe D, Bojkova D, Baek K, Bhattacharya A, Schulz L, Widera M, Mehdipour AR, Tascher G, Geurink PP, Wilhelm A, van der Heden van Noort GJ, Ovaa H, Müller S, Knobeloch KP, Rajalingam K, Schulman BA, Cinatl J, Hummer G, Ciesek S, Dikic I.
Nature. 2020 Nov 587(7835):657-662. doi: 10.1038/s41586-020-2601-5 Epub 2020 Jul 29
Gene expression and cell identity controlled by anaphase-promoting complex.
Oh E, Mark KG, Mocciaro A, Watson ER, Prabu JR, Cha DD, Kampmann M, Gamarra N, Rape M.
Nature. 2020 579(7797):136-140. doi: 10.1038/s41586-020-2034-1. Epub 2020 Feb 19
Quantifying the heterogeneity of macromolecular machines by mass photometry.
Sonn-Segev A, Belacic K, Bodrug T, Young G, VanderLinden RT, Schulman BA, SchimpfJ, Friedrich T, Dip PV Schwartz TU, Bauer B, Peters JM, Struw WB, Benesch JLP, Brown NG, Haselbach D, Kukura P.
Nat Commun 2020 Apr 14;11(1):1772. doi:10.1038/s41467-020-15642-w.
Paradoxical mitotic exit induced by a small molecule inhibitor of APC/CCdc20
Richeson KV, Bodrug T, Sackton KL, Yamaguchi M, Paulo JA, Gygi SP, Schulman BA, Brown NG, King RW,.
Nat Chem Biol. 2020 May;16(5):546-555. doi: 10.1038/s41589-020-0495-z. Epub 2020 Mar 9
NEDD8 nucleates a multivalent cullin-RING-UBE2D ubiquitin ligation assembly.
Baek K, Krist DT, Prabu JR, Hill S, Klügel M, Neumaier L-M, von Gronau S, Kleiger G, Schulman BA.
Nature. 2020 Feb;578(7795):461-466. doi: 10.1038/s41586-020-2000-y. Epub 2020 Feb 12
Molecular glue concept solidifies.
Baek K, Schulman BA.
Nat Chem Biol. 2020 Jan;16(1): 2-3. doi: 10.1038/s41589-019-0414-3
Allosteric regulation through a switch element in the autophagy E2, Atg3.
Qiu Y, Zheng Y, Grace CRR, Liu X, Klionsky DJ, Schulman BA.
Autophagy. 2020 Jan;16(1):183-184. doi: 10.1080/15548627.2019.1688550. Epub 2019 Nov 9
Interconversion between Anticipatory and Active GID E3 Ubiquitin Ligase Conformations via Metabolically Driven Substrate Receptor Assembly.
Qiao S, Langlois CR, Chrustowicz J, Sherpa D, Karayel O, Hansen FM, Beier V, von Gronau S, Bollschweiler D, Schäfer T, Alpi AF, Mann M, Prabu JR, Schulman BA.
Mol Cell. 2020 Jan 2;77(1):150-163.e9. doi:10.1016/j.molcel.2019.10.009. Epub 2019 Nov 7
2019
Robust cullin-RING ligase function is established by a multiplicity of poly-ubiquitylation pathways.
Hill S, Reichermeier K, Scott DC, Samentar L, Coulombe-Huntington J, Izzi L, Tang X, Ibarra R, Bertomeu T, Moradian A, Sweredoski MJ, Caberoy N, Schulman BA, Sicheri F, Tyers M, Kleiger G.
Elife e51163, 2019.
Discovery of Novel Pyrazolo-pyridone DCN1 Inhibitors Controlling Cullin Neddylation.
Kim HS, Hammill JT, Scott DC, Chen Y, Min J, Rector J, Singh B, Schulman BA, Guy RK.
J Med Chem. 2019 Sep 26;62(18):8429-8442. doi: 10.1021/acs.jmedchem.9b00410. Epub 2019 Sep 13
Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site.
Watson ER, Grace CRR, Zhang W, Miller DJ, Davidson IF, Prabu JR, Yu S, Bolhuis DL, Kulko ET, Vollrath R, Haselbach D, Stark H, Peters JM, Brown NG, Sidhu SS, Schulman BA.
Proc Natl Acad Sci USA. 2019 Aug 27;116(35):17280-17289. doi: 10.1073/pnas.1902889116. Epub 2019 Jul 26
A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade.
Zheng Y, Qiu Y, Grace CRR, Liu X, Klionsky DJ, Schulman BA.
Nat Commun. 2019 Aug 9;10(1):3600. doi: 10.1038/s41467-019-11435-y
ARIH2 is a Vif-dependent regulator of CUL5-mediated APOBEC3G degradation in HIV infection.
Hüttenhain R, Xu J, Burton LA, Gordon D, Hultquist JF, Johnson JR, Satkamp L, Hiatt J, Rhee DY, Baek K, Crosby DC, Frankel AD, Marson A, Harper WJ, Alpi AF, Schulman BA, Gross JD, Krogan NJ.
Cell Host and Microbe. 2019 Jul 10;26(1):86-99. doi: 10.1016/j.chom.2019.05.008. Epub 2019 Jun 25
Dual-color pulse-chase ubiquitination assays to simultaneously monitor substrate priming and extension.
Scott DC, Schulman BA.
Methods Enzymol. 2019 ;618:29-48. doi: 10.1016/bs.mie.2019.01.004. Epub 2019 Feb 11
Posing the APC/C E3 Ubiquitin Ligase to Orchestrate Cell Division.
Watson ER, Brown NG, Peters JM, Stark H, Schulman BA.
Trends Cell Biol. 2019 Feb 29;(2):117-134. doi: 10.1016/j.tcb.2018.09.007. Epub 2018 Oct 25
2018
Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation of Active, Phase-Separated Compartments.
Bouchard JJ, Otero JH, Scott DC, Szulc E, Martin EW, Sabri N, Granata D, Marzahn MR, Lindorff-Larsen K, Salvatella X, Schulman BA, Mittag T.
Mol Cell. 2018 Oct 4;72(1):19-36. doi: 10.1016/j.molcel.2018.08.027. Epub 2018 Sep 20.
Piperidinyl Ureas Chemically Control Defective in Cullin Neddylation 1 (DCN1)-Mediated Cullin Neddylation.
Hammill JT, Scott DC, Min J, Connelly MC, Holbrook G, Zhu F, Matheny A, Yang L, Singh B, Schulman BA, Guy RK. J
Med Chem. 2018 Apr 12;61(7):2680-2693. doi: 10.1021/acs.jmedchem.7b01277. Epub 2018 Mar 26.
Discovery of an Orally Bioavailable Inhibitor of Defective in Cullin Neddylation 1 (DCN1)-Mediated Cullin Neddylation.
Hammill JT, Bhasin D, Scott DC, Min J, Chen Y, Lu Y, Yang L, Kim HS, Connelly MC, Hammill C, Holbrook G, Jeffries C, Singh B, Schulman BA, Guy RK.
J Med Chem. 2018 Apr 12;61(7):2694-2706. doi: 10.1021/acs.jmedchem.7b01282. Epub 2018 Mar 26
SCF E3 Ligase Substrates Switch from CAN-D to Can-ubiquitylate.
Scott DC, Schulman BA.
Mol Cell. 2018 Mar 1;69(5):721-723. doi: 10.1016/j.molcel.2018.02.019.
2017
Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase.
Scott DC, Hammill JT, Min J, Rhee DY, Connelly M, Sviderskiy VO, Bhasin D, Chen Y, Ong SS, Chai SC, Goktug AN, Huang G, Monda JK, Low J, Kim HS, Paulo JA, Cannon JR, Shelat AA, Chen T, Kelsall IR, Alpi AF, Pagala V, Wang X, Peng J, Singh B, Harper JW, Schulman BA*, Guy RK*.
Nat Chem Biol. 2017 Aug 13;8:850-857. doi: 10.1038/nchembio.2386. Epub 2017 Jun 5
Structural Studies of HHARI/UbcH7∼Ub Reveal Unique E2∼Ub Conformational Restriction by RBR RING1.
Dove KK, Olszewski JL, Martino L, Duda DM, Wu XS, Miller DJ, Reiter KH, Rittinger K, Schulman BA*, Klevit RE*.
Structure. 2017 Jun 6;25(6):890-900.e5. doi: 10.1016/j.str.2017.04.013. Epub 2017 May 25
Preview by Dunkerley & Shaw: RBR Ubiquitin Transfer: Not Simply an "Open" and "Closed" Case?
Insights into links between autophagy and the ubiquitin system from the structure of LC3B bound to the LIR motif from the E3 ligase NEDD4.
Qiu Y, Zheng Y, Wu KP, Schulman BA.
Protein Sci. 2017 Aug 26(8):1674-1680. doi: 10.1002/pro.3186. Epub 2017 Jun 12
Production of Human ATG Proteins for Lipidation Assays.
Zheng Y, Qiu Y, Gunderson JE, Schulman BA.
Methods Enzymol. 2017;587:97-113. doi: 10.1016/bs.mie.2016.09.055. Epub 2016 Nov 11
Crystallographic Characterization of ATG Proteins and Their Interacting Partners.
Qiu Y, Zheng Y, Taherbhoy AM, Kaiser SE, Schulman BA.
Methods Enzymol. 2017;587:227-246. doi: 10.1016/bs.mie.2016.09.058. Epub 2016 Nov 9
2016
Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation.
Scott DC, Rhee DY, Duda DM, Kelsall IR, Olszewski JL, Paulo JA, de Jong A, Ovaa H, Alpi AF, Harper JW*, Schulman BA*.
Cell. 2016 Aug 25;166(5):1198-1214.e24. doi: 10.1016/j.cell.2016.07.027.
• Preview by Kleiger & Deshaies: Tag Team Ubiquitin Ligases.
Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation.
Yamaguchi M, VanderLinden R, Weissmann F, Qiao R, Dube P, Brown NG, Haselbach D, Zhang W, Sidhu SS, Peters JM*, Stark H*, Schulman BA*.
Mol Cell. 2016 Aug 18;63(4):593-607. doi: 10.1016/j.molcel.2016.07.003. Epub 2016 Aug 10
• News and Views by Morgan: Cell division: Mitotic regulation comes into focus.
Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.
Brown NG, VanderLinden R, Watson ER, Weissmann F, Ordureau A, Wu KP, Zhang W, Yu S, Mercredi PY, Harrison JS, Davidson IF, Qiao R, Lu Y, Dube P, Brunner MR, Grace CRR, Miller DJ, Haselbach D, Jarvis MA, Yamaguchi M, Yanishevski D, Petzold G, Sidhu SS, Kuhlman B, Kirschner MW, Harper JW, Peters JM*, Stark H*, Schulman BA*.
Cell. 2016 Jun 2;165(6):1440-1453. doi: 10.1016/j.cell.2016.05.037.
A cascading activity-based probe sequentially targets E1-E2-E3 ubiquitin enzymes.
Mulder MP, Witting K, Berlin I, Pruneda JN, Wu KP, Chang JG, Merkx R, Bialas J, Groettrup M, Vertegaal AC, Schulman BA, Komander D, Neefjes J, El Oualid F, Ovaa H.
Nat Chem Biol. 2016 Jul;12(7):523-30. doi: 10.1038/nchembio.2084. Epub 2016 May 16
Mechanism of APC/CCDC20 activation by mitotic phosphorylation.
Qiao R, Weissmann F, Yamaguchi M, Brown NG, VanderLinden R, Imre R, Jarvis MA, Brunner MR, Davidson IF, Litos G, Haselbach D, Mechtler K, Stark H*, Schulman BA*, Peters JM*.
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2570-8. doi: 10.1073/pnas.1604929113. Epub 2016 Apr 25
biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes.
Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown NG, Lampert F, Westermann S, Stark H, Schulman BA*, Peters JM*.
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 10.1073/pnas.1604935113. Epub 2016 Apr 25
A role of autophagy in spinocerebellar ataxia-Rare exception or general principle?
Burmeister M, Lee JH, Schulman BA, Yapici Z, Tolun A, Juhasz G, Li JZ, Klionsky DJ.
Autophagy. 2016 Jul 2;12(7):1208-9. doi: 10.1080/15548627.2016.1170266. Epub 2016 Apr 22
System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes.
Zhang W, Wu KP, Sartori MA, Kamadurai HB, Ordureau A, Jiang C, Mercredi PY, Murchie R, Hu J, Persaud A, Mukherjee M, Li N, Doye A, Walker JR, Sheng Y, Hao Z, Li Y, Brown KR, Lemichez E, Chen J, Tong Y, Harper JW, Moffat J, Rotin D, Schulman BA*, Sidhu SS*.
Mol Cell. 2016 Apr 7;62(1):121-36. doi: 10.1016/j.molcel.2016.02.005. Epub 2016 Mar 3
• Preview by Canadeo & Huibregtse: A Billion Ubiquitin Variants to Probe and Modulate the UPS.
Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay.
Kim M, Sandford E, Gatica D, Qiu Y, Liu X, Zheng Y, Schulman BA, Xu J, Semple I, Ro SH, Kim B, Mavioglu RN, Tolun A, Jipa A, Takats S, Karpati M, Li JZ, Yapici Z, Juhasz G, Lee JH, Klionsky DJ, Burmeister M.
Elife. 2016 Jan 26;5:e12245. doi: 10.7554/eLife.12245.
Multiple Weak Linear Motifs Enhance Recruitment and Processivity in SPOP-Mediated Substrate Ubiquitination.
Pierce WK, Grace CR, Lee J, Nourse A, Marzahn MR, Watson ER, High AA, Peng J, Schulman BA, Mittag T.
J Mol Biol. 2016 Mar 27;428(6):1256-71. doi: 10.1016/j.jmb.2015.10.002. Epub 2015 Oct 22
Measuring APC/C-Dependent Ubiquitylation In Vitro.
Jarvis MA, Brown NG, Watson ER, VanderLinden R, Schulman BA*, Peters JM*.
Methods Mol Biol. 2016;1342:287-303. doi: 10.1007/978-1-4939-2957-3_18.
2015
Itch WW Domains Inhibit Its E3 Ubiquitin Ligase Activity by Blocking E2-E3 Ligase Trans-thiolation.
Riling C, Kamadurai H, Kumar S, O'Leary CE, Wu KP, Manion EE, Ying M, Schulman BA, Oliver PM.
J Biol Chem. 2015 Sep 25;290(39):23875-87. doi: 10.1074/jbc.M115.649269.
ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space.
Chari A, Haselbach D, Kirves JM, Ohmer J, Paknia E, Fischer N, Ganichkin O, Möller V, Frye JJ, Petzold G, Jarvis M, Tietzel M, Grimm C, Peters JM, Schulman BA, Tittmann K, Markl J, Fischer U, Stark H.
Nat Methods. 2015 Sep;12(9):859-65. doi: 10.1038/nmeth.3493
Defining roles of PARKIN and ubiquitin phosphorylation by PINK1 in mitochondrial quality control using a ubiquitin replacement strategy.
Ordureau A, Heo JM, Duda DM, Paulo JA, Olszewski JL, Yanishevski D, Rinehart J, Schulman BA*, Harper JW*.
Proc Natl Acad Sci U S A. 2015 May 26;112(21):6637-42. doi: 10.1073/pnas.1506593112.
PAX5 is a tumor suppressor in mouse mutagenesis models of acute lymphoblastic leukemia.
Dang J, Wei L, de Ridder J, Su X, Rust AG, Roberts KG, Payne-Turner D, Cheng J, Ma J, Qu C, Wu G, Song G, Huether RG, Schulman B, Janke L, Zhang J, Downing JR, van der Weyden L, Adams DJ, Mullighan CG.
Blood. 2015 Jun 4;125(23):3609-17. doi: 10.1182/blood-2015-02-626127.
RING E3 mechanism for ubiquitin ligation to a disordered substrate visualized for human anaphase-promoting complex.
Brown NG, VanderLinden R, Watson ER, Qiao R, Grace CR, Yamaguchi M, Weissmann F, Frye JJ, Dube P, Ei Cho S, Actis ML, Rodrigues P, Fujii N, Peters JM*, Stark H*, Schulman BA*.
Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):5272-9. doi: 10.1073/pnas.1504161112.
Protein neddylation: beyond cullin-RING ligases.
Enchev RI, Schulman BA, Peter M.
Nat Rev Mol Cell Biol. 2015 Jan;16(1):30-44. doi: 10.1038/nrm3919.
Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome.
Yamaguchi M, Yu S, Qiao R, Weissmann F, Miller DJ, VanderLinden R, Brown NG, Frye JJ, Peters JM, Schulman BA.
J Mol Biol. 2015 Apr 24;427(8):1748-64. doi: 10.1016/j.jmb.2014.11.020.
2014
Mechanism of polyubiquitination by human anaphase-promoting complex: RING repurposing for ubiquitin chain assembly.
Brown NG, Watson ER, Weissmann F, Jarvis MA, VanderLinden R, Grace CR, Frye JJ, Qiao R, Dube P, Petzold G, Cho SE, Alsharif O, Bao J, Davidson IF, Zheng JJ, Nourse A, Kurinov I, Peters JM*, Stark H*, Schulman BA*.
Mol Cell. 2014 Oct 23;56(2):246-60. doi: 10.1016/j.molcel.2014.09.009.
• Preview by Iwai & Tanaka: Ubiquitin chain elongation: an intriguing strategy.
Quantitative proteomics reveal a feedforward mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis.
Ordureau A, Sarraf SA, Duda DM, Heo JM, Jedrychowski MP, Sviderskiy VO, Olszewski JL, Koerber JT, Xie T, Beausoleil SA, Wells JA, Gygi SP, Schulman BA, Harper JW.
Mol Cell. 2014 Nov 6;56(3):360-75. doi: 10.1016/j.molcel.2014.09.007.
• Preview by Stolz & Dikic: PINK1-PARKIN interplay: down to ubiquitin phosphorylation.
Structure of a RING E3 trapped in action reveals ligation mechanism for the ubiquitin-like protein NEDD8.
Scott DC, Sviderskiy VO, Monda JK, Lydeard JR, Cho SE, Harper JW, Schulman BA.
Cell. 2014 Jun 19;157(7):1671-84. doi: 10.1016/j.cell.2014.04.037.
The instability of the BTB-KELCH protein Gigaxonin causes Giant Axonal Neuropathy and constitutes a new penetrant and specific diagnostic test.
Boizot A, Talmat-Amar Y, Morrogh D, Kuntz NL, Halbert C, Chabrol B, Houlden H, Stojkovic T, Schulman BA, Rautenstrauss B, Bomont P.
Acta Neuropathol Commun. 2014 Apr 24;2:47. doi: 10.1186/2051-5960-2-47.
Atomistic autophagy: the structures of cellular self-digestion.
Hurley JH, Schulman BA.
Cell. 2014 Apr 10;157(2):300-311. doi: 10.1016/j.cell.2014.01.070.
Dynamic regulation of macroautophagy by distinctive ubiquitin-like proteins.
Klionsky DJ, Schulman BA.
Nat Struct Mol Biol. 2014 Apr;21(4):336-45. doi: 10.1038/nsmb.2787.
SPOP promotes tumorigenesis by acting as a key regulatory hub in kidney cancer.
Li G, Ci W, Karmakar S, Chen K, Dhar R, Fan Z, Guo Z, Zhang J, Ke Y, Wang L, Zhuang M, Hu S, Li X, Zhou L, Li X, Calabrese MF, Watson ER, Prasad SM, Rinker-Schaeffer C, Eggener SE, Stricker T, Tian Y, Schulman BA, Liu J, White KP.
Cancer Cell. 2014 Apr 14;25(4):455-68. doi: 10.1016/j.ccr.2014.02.007.
2013
Building and remodelling Cullin-RING E3 ubiquitin ligases.
Lydeard JR, Schulman BA, Harper JW.
EMBO Rep. 2013 Dec;14(12):1050-61. doi: 10.1038/embor.2013.173.
Binding to E1 and E3 is mutually exclusive for the human autophagy E2 Atg3.
Qiu Y, Hofmann K, Coats JE, Schulman BA, Kaiser SE.
Protein Sci. 2013 Dec;22(12):1691-7. doi: 10.1002/pro.2381.
TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes.
Kelsall IR, Duda DM, Olszewski JL, Hofmann K, Knebel A, Langevin F, Wood N, Wightman M, Schulman BA, Alpi AF.
EMBO J. 2013 Oct 30;32(21):2848-60. doi: 10.1038/emboj.2013.209.
Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3.
Kamadurai HB, Qiu Y, Deng A, Harrison JS, Macdonald C, Actis M, Rodrigues P, Miller DJ, Souphron J, Lewis SM, Kurinov I, Fujii N, Hammel M, Piper R, Kuhlman B, Schulman BA.
Elife. 2013 Aug 8;2:e00828. doi: 10.7554/eLife.00828.
• Insight by Meyer & Rape: Caught in the act.
Electron microscopy structure of human APC/C(CDH1)-EMI1 reveals multimodal mechanism of E3 ligase shutdown.
Frye JJ, Brown NG, Petzold G, Watson ER, Grace CR, Nourse A, Jarvis MA, Kriwacki RW, Peters JM*, Stark H*, Schulman BA*.
Nat Struct Mol Biol. 2013 Jul;20(7):827-35. doi: 10.1038/nsmb.2593.
• News & Views by Yamano: EMI1, a three-in-one ubiquitylation inhibitor.
• Leading Edge: Intrinsically disordered proteins, https://doi.org/10.1016/j.cell.2013.07.026
Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism.
Duda DM, Olszewski JL, Schuermann JP, Kurinov I, Miller DJ, Nourse A, Alpi AF, Schulman BA.
Structure. 2013 Jun 4;21(6):1030-41. doi: 10.1016/j.str.2013.04.019.
• Preview by Davies & Das: HHARI is one HECT of a RING.
• Have you seen? by Byrd & Weissman: Compact Parkin only: insights into the structure of an autoinhibited ubiquitin ligase.
Structures of Atg7-Atg3 and Atg7-Atg10 reveal noncanonical mechanisms of E2 recruitment by the autophagy E1.
Kaiser SE, Qiu Y, Coats JE, Mao K, Klionsky DJ, Schulman BA.
Autophagy. 2013 May;9(5):778-80. doi: 10.4161/auto.23644.
SCFFbxw5 mediates transient degradation of actin remodeller Eps8 to allow proper mitotic progression.
Werner A, Disanza A, Reifenberger N, Habeck G, Becker J, Calabrese M, Urlaub H, Lorenz H, Schulman B, Scita G, Melchior F.
Nat Cell Biol. 2013 Feb;15(2):179-88. doi: 10.1038/ncb2661.
Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes.
Monda JK, Scott DC, Miller DJ, Lydeard J, King D, Harper JW, Bennett EJ, Schulman BA.
Structure. 2013 Jan 8;21(1):42-53. doi: 10.1016/j.str.2012.10.013.
2012
Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures.
Kaiser SE, Mao K, Taherbhoy AM, Yu S, Olszewski JL, Duda DM, Kurinov I, Deng A, Fenn TD, Klionsky DJ, Schulman BA.
Nat Struct Mol Biol. 2012 Dec;19(12):1242-9. doi: 10.1038/nsmb.2415.
• Research Highlights by Donner: E2 loader, doi:10.1038/nchembio.1145
APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.
Uzunova K, Dye BT, Schutz H, Ladurner R, Petzold G, Toyoda Y, Jarvis MA, Brown NG, Poser I, Novatchkova M, Mechtler K, Hyman AA, Stark H, Schulman BA, Peters JM.
Nat Struct Mol Biol. 2012 Nov;19(11):1116-23. doi: 10.1038/nsmb.2412.
• News and Views by Musacchio & Ciliberto: The spindle-assembly checkpoint and the beauty of self-destruction.
Structural basis for a reciprocal regulation between SCF and CSN.
Enchev RI, Scott DC, da Fonseca PC, Schreiber A, Monda JK, Schulman BA*, Peter M*, Morris EP*.
Cell Rep. 2012 Sep 27;2(3):616-27. doi: 10.1016/j.celrep.2012.08.019.
Structural biology: A protein engagement RING.
Lima CD, Schulman BA.
Nature. 2012 Sep 6;489(7414):43-4. doi: 10.1038/489043a.
Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface.
Duda DM, Olszewski JL, Tron AE, Hammel M, Lambert LJ, Waddell MB, Mittag T, DeCaprio JA, Schulman BA.
Mol Cell. 2012 Aug 10;47(3):371-82. doi: 10.1016/j.molcel.2012.05.044.
• Preview by Hristova, Stringer & Weissman: Cullin RING ligases: glommed by glomulin.
Ubiquitin-like modifiers.
Taherbhoy AM, Schulman BA, Kaiser SE.
Essays Biochem. 2012;52:51-63. doi: 10.1042/bse0520051.
The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7.
Tron AE, Arai T, Duda DM, Kuwabara H, Olszewski JL, Fujiwara Y, Bahamon BN, Signoretti S, Schulman BA, DeCaprio JA.
Mol Cell. 2012 Apr 13;46(1):67-78. doi: 10.1016/j.molcel.2012.02.005.
Viral E3 ubiquitin ligase-mediated degradation of a cellular E3: viral mimicry of a cellular phosphorylation mark targets the RNF8 FHA domain.
Chaurushiya MS, Lilley CE, Aslanian A, Meisenhelder J, Scott DC, Landry S, Ticau S, Boutell C, Yates JR 3rd, Schulman BA, Hunter T, Weitzman MD.
Mol Cell. 2012 Apr 13;46(1):79-90. doi: 10.1016/j.molcel.2012.02.004.
Trans mechanism for ubiquitin-like protein transfer in autophagy.
Taherbhoy AM, Kaiser SE, Schulman BA.
Cell Cycle. 2012 Feb 15;11(4):635-6. doi: 10.4161/cc.11.4.19277.
2011
Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway.
Taherbhoy AM, Tait SW, Kaiser SE, Williams AH, Deng A, Nourse A, Hammel M, Kurinov I, Rock CO, Green DR, Schulman BA.
Mol Cell. 2011 Nov 4;44(3):451-61. doi: 10.1016/j.molcel.2011.08.034.
Twists and turns in ubiquitin-like protein conjugation cascades.
Schulman BA.
Protein Sci. 2011 Dec;20(12):1941-54. doi: 10.1002/pro.750.
N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex.
Scott DC, Monda JK, Bennett EJ, Harper JW, Schulman BA.
Science. 2011 Nov 4;334(6056):674-8. doi: 10.1126/science.1209307.
• Research Highlights by Mason: Acetylating for avidity, doi:10.1038/nsmb.2181
A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases.
Calabrese MF, Scott DC, Duda DM, Grace CR, Kurinov I, Kriwacki RW, Schulman BA.
Nat Struct Mol Biol. 2011 Jul 17;18(8):947-9. doi: 10.1038/nsmb.2086.
• News and Views by Rahighi & Dikic: Conformational flexibility and rotation of the RING domain in activation of cullin-RING ligases.
Structural regulation of cullin-RING ubiquitin ligase complexes.
Duda DM, Scott DC, Calabrese MF, Zimmerman ES, Zheng N*, Schulman BA*.
Curr Opin Struct Biol. 2011 Apr;21(2):257-64. doi: 10.1016/j.sbi.2011.01.003.
IDH1 and IDH2 mutations in pediatric acute leukemia.
Andersson AK, Miller DW, Lynch JA, Lemoff AS, Cai Z, Pounds SB, Radtke I, Yan B, Schuetz JD, Rubnitz JE, Ribeiro RC, Raimondi SC, Zhang J, Mullighan CG, Shurtleff SA, Schulman BA, DowningJR.
Leukemia. 2011 Oct;25(10):1570-7. doi: 10.1038/leu.2011.133.
2010
Crystal structure of UBA2(ufd)-Ubc9: insights into E1-E2 interactions in Sumo pathways.
Wang J, Taherbhoy AM, Hunt HW, Seyedin SN, Miller DW, Miller DJ, Huang DT, Schulman BA.
PLoS One. 2010 Dec 30;5(12):e15805. doi: 10.1371/journal.pone.0015805.
Pathogenic bacteria target NEDD8-conjugated cullins to hijack host-cell signaling pathways.
Jubelin G, Taieb F, Duda DM, Hsu Y, Samba-Louaka A, Nobe R, Penary M, Watrin C, Nougayrède JP, Schulman BA, Stebbins CE, Oswald E.
PLoS Pathog. 2010 Sep 30;6(9):e1001128. doi: 10.1371/journal.ppat.1001128.
Structural assembly of cullin-RING ubiquitin ligase complexes.
Zimmerman ES, Schulman BA, Zheng N.
Curr Opin Struct Biol. 2010 Dec;20(6):714-21. doi: 10.1016/j.sbi.2010.08.010.
A dual E3 mechanism for Rub1 ligation to Cdc53.
Scott DC, Monda JK, Grace CR, Duda DM, Kriwacki RW, Kurz T, Schulman BA.
Mol Cell. 2010 Sep 10;39(5):784-96. doi: 10.1016/j.molcel.2010.08.030.
Structural biology: Transformative encounters.
Schulman BA, Haas AL.
Nature. 2010 Feb 18;463(7283):889-90. doi: 10.1038/463889a.
Identification and characterization of the first small molecule inhibitor of MDMX.
Reed D, Shen Y, Shelat AA, Arnold LA, Ferreira AM, Zhu F, Mills N, Smithson DC, Regni CA, Bashford D, Cicero SA, Schulman BA, Jochemsen AG, Guy RK, Dyer MA.
J Biol Chem. 2010 Apr 2;285(14):10786-96. doi: 10.1074/jbc.M109.056747.
2009
Insights into ubiquitin transfer cascades from a structure of a UbcH5B~ubiquitin-HECT(NEDD4L) complex.
Kamadurai HB, Souphron J, Scott DC, Duda DM, Miller DJ, Stringer D, Piper RC, Schulman BA.
Mol Cell. 2009 Dec 25;36(6):1095-102. doi: 10.1016/j.molcel.2009.11.010.
Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae.
Siergiejuk E, Scott DC, Schulman BA, Hofmann K, Kurz T, Peter M.
EMBO J. 2009 Dec 16;28(24):3845-56. doi: 10.1038/emboj.2009.354.
Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA.
Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure.
Wang J, Dye BT, Rajashankar KR, Kurinov I, Schulman BA.
Nat Struct Mol Biol. 2009 Sep;16(9):987-9. doi: 10.1038/nsmb.1645.
(G2)BRinging an E2 to E3.
Wang J, Schulman BA.
Structure. 2009 Jul 15;17(7):916-7. doi: 10.1016/j.str.2009.06.005.
How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic.
Regni CA, Roush RF, Miller DJ, Nourse A, Walsh CT, Schulman BA.
EMBO J. 2009 Jul 8;28(13):1953-64. doi: 10.1038/emboj.2009.146.
JAK mutations in high-risk childhood acute lymphoblastic leukemia.
Mullighan CG, Zhang J, Harvey RC, Collins-Underwood JR, Schulman BA, Phillips LA, Tasian SK, Loh ML, Su X, Liu W, Devidas M, Atlas SR, Chen IM, Clifford RJ, Gerhard DS, Carroll WL, Reaman GH, Smith M, Downing JR, Hunger SP, Willman CL.
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9414-8. doi: 10.1073/pnas.0811761106.
Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways.
Schulman BA, Harper JW.
Nat Rev Mol Cell Biol. 2009 May;10(5):319-31. doi: 10.1038/nrm2673.
E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF*, Schulman BA*.
Mol Cell. 2009 Feb 27;33(4):483-95. doi: 10.1016/j.molcel.2009.01.011.
• Preview by Pichler: A second E2 for nedd8ylation expands substrate selection.
Deletion of IKZF1 and prognosis in acute lymphoblastic leukemia.
Mullighan CG, Su X, Zhang J, Radtke I, Phillips LA, Miller CB, Ma J, Liu W, Cheng C, Schulman BA, Harvey RC, Chen IM, Clifford RJ, Carroll WL, Reaman G, Bowman WP, Devidas M, Gerhard DS, Yang W, Relling MV, Shurtleff SA, Campana D, Borowitz MJ, Pui CH, Smith M, Hunger SP, Willman CL, Downing JR; Children's Oncology Group.
N Engl J Med. 2009 Jan 29;360(5):470-80. doi: 10.1056/NEJMoa0808253.
2008
Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation.
Duda DM, Borg LA, Scott DC, Hunt HW, Hammel M, Schulman BA.
Cell. 2008 Sep 19;134(6):995-1006. doi: 10.1016/j.cell.2008.07.022.
• Preview by Saifee & Zheng: A ubiquitin-like protein unleashes ubiquitin ligases.
Structural dissection of a gating mechanism preventing misactivation of ubiquitin by NEDD8's E1.
Souphron J, Waddell MB, Paydar A, Tokgöz-Gromley Z, Roussel MF, Schulman BA.
Biochemistry. 2008 Aug 26;47(34):8961-9. doi: 10.1021/bi800604c.
Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2.
Huang DT, Zhuang M, Ayrault O, Schulman BA.
Nat Struct Mol Biol. 2008 Mar;15(3):280-7. doi: 10.1038/nsmb.1387.
2007
Structural mechanisms underlying posttranslational modification by ubiquitin-like proteins.
Dye BT, Schulman BA.
Annu Rev Biophys Biomol Struct. 2007;36:131-50.
Structure of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p: conservation of a non-covalent ubiquitin-like protein-E2 complex as a platform for selective interactions within a SUMO pathway.
Duda DM, van Waardenburg RC, Borg LA, McGarity S, Nourse A, Waddell MB, Bjornsti MA, Schulman BA.
J Mol Biol. 2007 Jun 8;369(3):619-30.
Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity.
Huang DT, Hunt HW, Zhuang M, Ohi MD, Holton JM, Schulman BA.
Nature. 2007 Jan 25;445(7126):394-8.
• News and Views by Trempe & Endicott: Structural biology: pass the protein.
• Preview by Haas: Structural insights into early events in the conjugation of ubiquitin and ubiquitin-like proteins.
2006
Breaking up with a kinky SUMO.
Huang DT, Schulman BA.
Nat Struct Mol Biol. 2006 Dec;13(12):1045-7.
Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress.
van Waardenburg RC, Duda DM, Lancaster CS, Schulman BA, Bjornsti MA.
Mol Cell Biol. 2006 Jul;26(13):4958-69.
Structural complexity in ubiquitin recognition.
Harper JW, Schulman BA.
Cell. 2006 Mar 24;124(6):1133-6.
A calcium-regulated MEF2 sumoylation switch controls postsynaptic differentiation.
Shalizi A, Gaudillière B, Yuan Z, Stegmüller J, Shirogane T, Ge Q, Tan Y, Schulman B, Harper JW, Bonni A.
Science. 2006 Feb 17;311(5763):1012-7.
2005
Protein ubiquitination: CHIPping away the symmetry.
Schulman BA, Chen ZJ.
Mol Cell. 2005 Dec 9;20(5):653-5.
High-level expression and purification of recombinant SCF ubiquitin ligases.
Li T, Pavletich NP*, Schulman BA*, Zheng N*.
Methods Enzymol. 2005;398:125-42.
Expression, purification, and characterization of the E1 for human NEDD8, the heterodimeric APPBP1-UBA3 complex.
Huang DT, Schulman BA.
Methods Enzymol. 2005;398:9-20.
Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase.
Hao B, Zheng N, Schulman BA, Wu G, Miller JJ, Pagano M, Pavletich NP.
Mol Cell. 2005 Oct 7;20(1):9-19.
E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer.
Eletr ZM, Huang DT, Duda DM, Schulman BA, Kuhlman B.
Nat Struct Mol Biol. 2005 Oct;12(10):933-4.
Tag-team SUMO wrestling.
Duda DM, Schulman BA.
Mol Cell. 2005 Jun 10;18(6):612-4.
Structural analysis of Escherichia coli ThiF.
Duda DM, Walden H, Sfondouris J, Schulman BA.
J Mol Biol. 2005 Jun 17;349(4):774-86.
Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1.
Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA.
Mol Cell. 2005 Feb 4;17(3):341-50.
• Preview by VanDemark & Hill: E1 on the move.
2004
A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8.
Huang DT, Miller DW, Mathew R, Cassell R, Holton JM, Roussel MF, Schulman BA.
Nat Struct Mol Biol. 2004 Oct;11(10):927-35.
• Preview by VanDemark & Hill: Grabbing E2 by the tail.
Structural and dynamic independence of isopeptide-linked RanGAP1 and SUMO-1.
Macauley MS, Errington WJ, Okon M, Schärpf M, Mackereth CD, Schulman BA, McIntosh LP.
J Biol Chem. 2004 Nov 19;279(47):49131-7.
The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein.
Zhao C, Beaudenon SL, Kelley ML, Waddell MB, Yuan W, Schulman BA, Huibregtse JM, Krug RM.
Proc Natl Acad Sci U S A. 2004 May 18;101(20):7578-82.
Local and global cooperativity in the human alpha-lactalbumin molten globule.
Quezada CM, Schulman BA, Froggatt JJ, Dobson CM, Redfield C.
J Mol Biol. 2004 Apr 16;338(1):149-58.
Ubiquitin-like protein activation.
Huang DT, Walden H, Duda D, Schulman BA.
Oncogene. 2004 Mar 15;23(11):1958-71.
2003
The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1.
Walden H, Podgorski MS, Huang DT, Miller DW, Howard RJ, Minor DL Jr, Holton JM, Schulman BA.
Mol Cell. 2003 Dec;12(6):1427-37.
Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase.
Wu G, Xu G, Schulman BA, Jeffrey PD, Harper JW, Pavletich NP.
Mol Cell. 2003 Jun;11(6):1445-56.
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA.
Nature. 2003 Mar 20;422(6929):330-4.
• News and Views by VanDemark & Hill: Two-stepping with E1.
Parkin is a component of an SCF-like ubiquitin ligase complex and protects postmitotic neurons from kainate excitotoxicity.
Staropoli JF, McDermott C, Martinat C, Schulman B, Demireva E, Abeliovich A.
Neuron. 2003 Mar 6;37(5):735-49.
2002
Identification of a multifunctional binding site on Ubc9p required for Smt3p conjugation.
Bencsath KP, Podgorski MS, Pagala VR, Slaughter CA, Schulman BA.
J Biol Chem. 2002 Dec 6;277(49):47938-45.
The SOCS box: a tale of destruction and degradation.
Kile BT, Schulman BA, Alexander WS, Nicola NA, Martin HM, Hilton DJ.
Trends Biochem Sci. 2002 May;27(5):235-41.
Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex.
Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P, Chu C, Koepp DM, Elledge SJ, Pagano M, Conaway RC, Conaway JW, Harper JW, Pavletich NP.
Nature. 2002 Apr 18;416(6882):703-9.
2001 and earlier
Probing subtle differences in the hydrogen exchange behavior of variants of the human alpha-lactalbumin molten globule using mass spectrometry.
Last AM, Schulman BA, Robinson CV, Redfield C.
J Mol Biol. 2001 Aug 24;311(4):909-19.
A splice variant of Skp2 is retained in the cytoplasm and fails to direct cyclin D1 ubiquitination in the uterine cancer cell line SK-UT.
Ganiatsas S, Dow R, Thompson A, Schulman B, Germain D.
Oncogene. 2001 Jun 21;20(28):3641-50.
Role of the F-box protein Skp2 in lymphomagenesis.
Latres E, Chiarle R, Schulman BA, Pavletich NP, Pellicer A, Inghirami G, Pagano M.
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2515-20.
Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex.
Schulman BA, Carrano AC, Jeffrey PD, Bowen Z, Kinnucan ER, Finnin MS, Elledge SJ, Harper JW, Pagano M, Pavletich NP.
Nature. 2000 Nov 16;408(6810):381-6.
Alpha-lactalbumin forms a compact molten globule in the absence of disulfide bonds.
Redfield C, Schulman BA, Milhollen MA, Kim PS, Dobson CM.
Nat Struct Biol. 1999 Oct;6(10):948-52.
Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A.
Schulman BA, Lindstrom DL, Harlow E.
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10453-8.
A residue-specific NMR view of the non-cooperative unfolding of a molten globule.
Schulman BA, Kim PS, Dobson CM, Redfield C.
Nat Struct Biol. 1997 Aug;4(8):630-4.
Proline scanning mutagenesis of a molten globule reveals non-cooperative formation of a protein's overall topology.
Schulman BA, Kim PS.
Nat Struct Biol. 1996 Aug;3(8):682-7.
Disulfide determinants of calcium-induced packing in alpha-lactalbumin.
Wu LC, Schulman BA, Peng ZY, Kim PS.
Biochemistry. 1996 Jan 23;35(3):859-63.
Different subdomains are most protected from hydrogen exchange in the molten globule and native states of human alpha-lactalbumin.
Schulman BA, Redfield C, Peng ZY, Dobson CM, Kim PS.
J Mol Biol. 1995 Nov 10;253(5):651-7.
Does the molten globule have a native-like tertiary fold?
Peng ZY, Wu LC, Schulman BA, Kim PS.
Philos Trans R Soc Lond B Biol Sci. 1995 Apr 29;348(1323):43-7.
Hydrogen exchange in BPTI variants that do not share a common disulfide bond.
Schulman BA, Kim PS.
Protein Sci. 1994 Dec;3(12):2226-32.
Hydrogen-oxidizing electron transport components in the hyperthermophilic archaebacterium Pyrodictium brockii.
Pihl TD, Black LK, Schulman BA, Maier RJ.
J Bacteriol. 1992 Jan;174(1):137-43.
Hydrogen-sulfur autotrophy in the hyperthermophilic archaebacterium, Pyrodictium brockii.
Pihl TD, Schicho RN, Black LK, Schulman BA, Maier RJ, Kelly RM.
Biotechnol Genet Eng Rev. 1990;8:345-77.