Anastasija Panevska
PostDoc
After completing her Bachelor's degree in Biology and a Master's in Molecular and Functional Biology, Anastasija obtained her PhD in Molecular Biology and Biochemistry at the Medical Faculty, University of Ljubljana. During her PhD she focused on studying pore-forming proteins and their interactions with lipid membranes. In 2023, she was awarded a Humboldt Research Fellowship and joined the lab of Petra Schwille. Currently, her work is centered on exploring FtsZ-driven synthetic division within lipid vesicles, aiming to unravel the mechanisms of cellular division and its implications for synthetic biology.
Research Interests
Bottom-up Synthetic Biology, in vitro Reconstitution, bacterial division proteins, protein-lipid interaction
Publications
Original scientific article
Gambardella C, Miroglio R, Trenti F, Guella G, Panevska A, Sbrana F, Grunder M, Garaventa F, Sepčić K. Assessing the toxicity of aegerolysin-based bioinsecticidal complexes using the sea urchin Paracentrotus lividus as model organism. Aquat Toxicol. 2023 Nov;264:106727. doi: 10.1016/j.aquatox.2023.106727.
Yilmaz N, Panevska A, Tomishige N, Richert L, Mély Y, Sepčić K, Greimel P, Kobayashi T. Assembly dynamics and structure of an aegerolysin, ostreolysin A6. J Biol Chem. 2023 Aug;299(8):104940. doi: 10.1016/j.jbc.2023.104940.
Panevska A, Čegovnik N, Fortuna K, Vukovič A, Grundner M, Modic Š, Bajc G, Skočaj M, Mravinec Bohte M, Popošek LL, Žigon P, Razinger J, Veranič P, Resnik N, Sepčić K. A single point mutation expands the applicability of ostreolysin A6 in biomedicine. Sci Rep. 2023 Feb 7;13(1):2149. doi: 10.1038/s41598-023-28949-7
Balbi T, Trenti F, Panevska A, Bajc G, Guella G, Ciacci C, Canonico B, Canesi L, Sepčić K. Ceramide Aminoethylphosphonate as a New Molecular Target for Pore-Forming Aegerolysin-Based Protein Complexes. Front Mol Biosci. 2022 May 25;9:902706. doi: 10.3389/fmolb.2022.902706.
Kočar E, Lenarčič T, Hodnik V, Panevska A, Huang Y, Bajc G, Kostanjšek R, Naren AP, Maček P, Anderluh G, Sepčić K, Podobnik M, Butala M. Crystal structure of RahU, an aegerolysin protein from the human pathogen Pseudomonas aeruginosa, and its interaction with membrane ceramide phosphorylethanolamine. Sci Rep. 2021 Mar 22;11(1):6572. doi: 10.1038/s41598-021-85956-2.
Kraševec N, Panevska A, Lemež Š, Razinger J, Sepčić K, Anderluh G, Podobnik M. Lipid-Binding Aegerolysin from Biocontrol Fungus Beauveria bassiana. Toxins (Basel). 2021 Nov 20;13(11):820. doi: 10.3390/toxins13110820.
Panevska A, Glavan G, Jemec Kokalj A, Kukuljan V, Trobec T, Žužek MC, Vrecl M, Drobne D, Frangež R, Sepčić K. Effects of Bioinsecticidal Aegerolysin-Based Cytolytic Complexes on Non-Target Organisms. Toxins (Basel). 2021 Jun 30;13(7):457. doi: 10.3390/toxins13070457.
Milijaš Jotić M, Panevska A, Iacovache I, Kostanjšek R, Mravinec M, Skočaj M, Zuber B, Pavšič A, Razinger J, Modic Š, Trenti F, Guella G, Sepčić K. Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm. Toxins (Basel). 2021 Jun 29;13(7):455. doi: 10.3390/toxins13070455.
Novak M, Krpan T, Panevska A, Shewell LK, Day CJ, Jennings MP, Guella G, Sepčić K. Binding specificity of ostreolysin A6 towards Sf9 insect cell lipids. Biochim Biophys Acta Biomembr. 2020 Sep 1;1862(9):183307. doi: 10.1016/j.bbamem.2020.183307.
Panevska A, Hodnik V, Skočaj M, Novak M, Modic Š, Pavlic I, Podržaj S, Zarić M, Resnik N, Maček P, Veranič P, Razinger J, Sepčić K. Pore-forming protein complexes from Pleurotus mushrooms kill western corn rootworm and Colorado potato beetle through targeting membrane ceramide phosphoethanolamine. Sci Rep. 2019 Mar 25;9(1):5073. doi: 10.1038/s41598-019-41450-4.
Review article
Panevska A, Skočaj M, Modic Š, Razinger J, Sepčić K. Aegerolysins from the fungal genus Pleurotus - Bioinsecticidal proteins with multiple potential applications. J Invertebr Pathol. 2021 Nov;186:107474. doi: 10.1016/j.jip.2020.107474.
Grundner M, Panevska A, Sepčić K, Skočaj M. What Can Mushroom Proteins Teach Us about Lipid Rafts? Membranes (Basel). 2021 Apr 6;11(4):264. doi: 10.3390/membranes11040264.
Panevska A, Skočaj M, Križaj I, Maček P, Sepčić K. Ceramide phosphoethanolamine, an enigmatic cellular membrane sphingolipid. Biochim Biophys Acta Biomembr. 2019 Jul 1;1861(7):1284-1292. doi: 10.1016/j.bbamem.2019.05.001.
Patent
PANEVSKA, Anastasija, RAZINGER, Jaka, SEPČIĆ, Kristina, MAČEK, Peter, SKOČAJ, Matej, MODIC, Špela, NOVAK, Maruša, BUTALA, Matej, HODNIK, Vesna, GRUNDNER, Maja, TURK, Tom. New bio-pesticides for controlling plant pests : European patent specification EP 3 662 069 B1, 2023-08-30 = Neue Biopestizide zur Bekämpfung von Pflanzenschädlingen = Nouveaux biopesticides pour lutter contre les parasites des plantes. Munich: European Patent Office, 2023. patent family: EP3662069A1, 2020-06-10; EP2017074877W, 2017-09-29; CA3077263A1, 2019-04-04; CN111164212A, 2020-05-15; US2020245628A1, 2020-08-06; BR112020005331A2, 2020-09-24; WO2019063101A1, 2019-04-04
Patent application
SEPČIĆ, Kristina, GRUNDNER, Maja, PANEVSKA, Anastasija, GRUDEN, Kristina, LUKAN, Tjaša, POGAČAR, Karmen, COLL RIUS, Anna, PETEK, Marko. Plants producing proteins for pest control : application number LU504095. Luxembourg: Office de la propriété intellectuelle Ministère de lʹÉconomie, 2023.