Matthias Mann

Contact

Prof. Dr. Matthias Mann

Director

Phone:+49 89 8578-2557Fax:+49 89 8578-2412

Email:mmann@...

MPI of Biochemistry

Google scholar

Einführung für interessierte

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events

10th MaxQuant Summer School

July 8th - 13th, 2018 CRG + CCCB (Barcelona, Spain) [more]

Tutorials

Video Tutorial

How to build a stage tipping syringe

Department of Proteomics and Signal Transduction

Matthias Mann Lab

ABOUT US

The department of proteomics and signal transduction develops and applies methods of mass spectrometry (MS)-based proteomics in a variety of biological areas.

It is led by Matthias Mann, who moved here in 2005 from earlier stations at the European Molecular Biology Laboratory (EMBL) and from the University of Southern Denmark (SDU). He is also affiliated with the NNF Center for Protein Research (University of Copenhagen).

NEWS

9th MaxQuant Summer School

A social network of human proteins

Eavesdropping on Immune Cells

Stage Tipping Video Tutorials

A protein atlas of the brain

Social network architecture of human immune cells

High-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform

Nature Protocols | Published: 06 September 2018
http://dx.doi.org/10.1038/s41596-018-0014-9

Sean J. Humphrey, Ozge Karayel, David E. James and Matthias Mann [more]

In vivo brain GPCR signaling elucidated by phosphoproteomics

Jeffrey J. Liu, Kirti Sharma, Luca Zangrandi, Chongguang Chen, Sean J. Humphrey, Yi-Ting Chiu, Mariana Spetea, Lee-Yuan Liu-Chen, Christoph Schwarzer, Matthias Mann

Science 22 Jun 2018:
Vol. 360, Issue 6395, eaao4927
DOI: 10.1126/science.aao4927
[Full text]

Rapid proteomic analysis for solid tumors reveals LSD1 as a drug target in an end‐stage cancer patient

Sophia Doll, Maximilian C. Kriegmair, Alberto Santos, Michael Wierer, Fabian Coscia, Helen Michele Neil, Stefan Porubsky, Philipp E. Geyer, Andreas Mund, Philipp Nuhn, Matthias Mann [more]

Online parallel accumulation − serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer

Florian Meier, Andreas-David Brunner, Scarlet Koch, Heiner Koch, Markus Lubeck, Michael Krause, Niels Goedecke, Jens Decker, Thomas Kosinski, Melvin A Park, Nicolai Bache, Ole Hoerning, Juergen Cox, Oliver Raether, Matthias Mann

A novel LC system embeds analytes in pre-formed gradients for rapid, ultra-robust proteomics

Nicolai Bache, Philipp E. Geyer, Dorte B. Bekker-Jensen, Ole Hoerning, Lasse Falkenby, Peter V. Treit, Sophia Doll, Igor Paron, Florian Meier, Jesper V. Olsen, Ole Vorm, Matthias Mann [more]

BoxCar acquisition method enables single-shot proteomics at a depth of 10,000 proteins in 100 minutes

Nature Methods (2018) doi:10.1038/s41592-018-0003-5

Meier F, Geyer PE, Winter SV, Cox J & Mann M [more]

Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis

Elife. 2017 Nov 10;6. pii: e31012. doi: 10.7554/eLife.31012.

Steger M, Diez F, Dhekne HS, Lis P, Nirujogi RS, Karayel O, Tonelli F, Martinez TN, Lorentzen E, Pfeffer SR, Alessi DR, Mann M.

A Primer on Concepts and Applications of Proteomics in Neuroscience

Neuron Volume 96, Issue 3, 1 November 2017, Pages 558–571;
https://doi.org/10.1016/j.neuron.2017.09.025

Fabian Hosp and Matthias Mann [more]

First German researcher, Matthias Mann, has reached an h-index higher than 200 - Congrats!

schoolar.google.com
pnas.org

Matthias Mann talks about clinical proteomics in interview with Evosep Biosystems

Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging

Cell Rep. 2017 Jun 13;19(11):2396-2409. doi: 10.1016/j.celrep.2017.05.054.

Murgia M, Toniolo L, Nagaraj N, Ciciliot S, Vindigni V, Schiaffino S, Reggiani C, Mann M.

Highlights
•Single-fiber proteomic analysis of human muscle aging
•Oxidative phosphorylation decreases similarly in slow and fast fibers
•Enzymes of carbohydrate metabolism increase in slow and decrease in fast aging fibers
•Proteomics elucidates why fast fibers age more rapidly than slow ones

Novo Nordisk Foundation Center for Protein Research

Olsen Group defines the HeLa cell proteome

Bekker-Jensen et al. show that proteomics can now provide an essentially complete HeLa proteome. They provide measurements acquired simultaneously for more than 12,200 protein-coding genes, 10,000 phosphorylation sites, and 7,000 N-acetylation sites. Their approach is fast, accessible, and requires modest amounts of starting material, making it easily extendable to other human cell lines and patient samples. - Bekker-Jensen et al., 2017, Cell Systems 4, 1–13 June 28, 2017

EMBL Alumni Award 2017
Matthias Mann winner of Lennart Philipson Award

“Matthias Mann, former Group Leader in EMBL’s Instrumentation Programme (1992-1998), has been selected as the 2017 Lennart Philipson Award winner for his pioneering work in the field of mass spectrometry-based proteomics.

Matthias has contributed breakthroughs that have revolutionised proteomics techniques in biomedical laboratories around the world. His contributions include the “peptide sequence tag” approach which allows proteins to be computationally identified by the fragment spectra; nanoelectrospray which allows minute quantities of protein to be tested; and methods for analysing proteins after extracting them from gels."

Social network architecture of human immune cells unveiled by quantitative proteomics

Jan C Rieckmann, Roger Geiger, Daniel Hornburg, Tobias Wolf, Ksenya Kveler, David Jarrossay, Federica Sallusto, Shai S Shen-Orr, Antonio Lanzavecchia, Matthias Mann & Felix Meissner

Immune cells give rise to the most interconnected system in the body. Here, we perform comprehensive proteomics and secretomics (http://immprot.org/) to describe in detail the 'social network' of human immune cells and throw light on novel cell connectivities

Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology

Cell Metabolism 25, 1–10January 10, 2017 ª 2016 Elsevier Inc.

Robles MS, Humphrey SJ, Mann M.

NATURE | INSIGHT | REVIEW

Mass-spectrometric exploration of proteome structure and function

Ruedi Aebersold & Matthias Mann

Nature 537, 347–355 (15 September 2016) doi:10.1038/nature19949
Received 11 January 2016 Accepted 15 July 2016 Published online 14 September 2016

Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status

Nat Commun. 2016 Aug 26;7:12645. doi: 10.1038/ncomms12645.

Coscia F, Watters KM, Curtis M, Eckert MA, Chiang CY, Tyanova S, Montag A, Lastra RR, Lengyel E, Mann M.

Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins

Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1673-82. doi: 10.1073/pnas.1519650113. Epub 2016 Feb 9.

Neufeld-Cohen A, Robles MS, Aviram R, Manella G, Adamovich Y, Ladeuix B, Nir D, Rousso-Noori L, Kuperman Y, Golik M, Mann M, Asher G

Highlighted at www.pnas.org

Cell Systems: featured article

Plasma Proteome Profiling to Assess Human Health and Disease

cell systems, Volume 2, Issue 3, p185–195, 23 March 2016
Geyer et al.

Preview

Deep Proteomics of Breast Cancer Cells Reveals that Metformin Rewires Signaling Networks Away from a Pro-growth State

Cell Systems, Volume 2, Issue 3, p142-143, 23 March 2016

Sacco et al.

Breakthrough in Parkinson’s Research

Identification of bona fide and verifiable substrates of the Parkinson’s disease kinase LRRK2 has eluded researchers for over ten years and was considered as one of the greatest challenges in the field. Using phosphoproteomics, we identified and validated Rab GTPases as key physiological LRRK2 substrates.

Phosphoproteomics reveals that Parkinson’s disease kinase LRRK2 regulates a subset of Rab GTPases

eLife, January 28, 2016
http://dx.doi.org/10.7554/eLife.12813

Steger, M et al.

Press Release
Dispatch in Current Biology
Comment in Movement Disorders

Matthias Mann receives The Order of Dannebrog Knights Cross

Her Majesty the Queen of Denmark recognizes Matthias for his outstanding contribution to mankind in general, and science in particular. [more]

Matthias Mann awarded Inaugural Barry L. Karger Medal on October 16, 2015 at Northeastern University, Boston , US.

the Medal is a biennial award recognizing an individual who has significantly contributed to the development of new bioanalytical methods. [more]

Parallel accumulation – serial fragmentation (PASEF): Multiplying sequencing speed and sensitivity by synchronized scans in a trapped ion mobility device

J. Proteome Res., November 5, 2015

Meier F, Beck S, Grassl N, Lubeck M, Park MA, Raether O, Mann M.

Cell type– and brain region–resolved mouse brain proteome

Nat Neurosci. 2015 Nov 2. doi: 10.1038/nn.4160

Sharma K, Schmitt S, Bergner CG, Tyanova S, Kannaiyan N, Manrique-Hoyos N, Kongi K, Cantuti L, Hanisch UK, Philips MA, Rossner MJ, Mann M, Simons M

Press Release

A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances

Cell 163(3):712-23 (2015). doi: 10.1016/j.cell.2015.09.053

Marco Y. Hein, Nina C. Hubner, Ina Poser, Jürgen Cox, Nagarjuna Nagaraj, Yusuke Toyoda, Igor A. Gak, Ina Weisswange, Jörg Mansfeld, Frank Buchholz, Anthony A. Hyman & Matthias Mann

Press Release

Research Highlight in Nature Reviews Molecular Cell Biology

High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics

Nat Biotechnol. 2015 Aug 17. doi: 10.1038/nbt.3327.

Humphrey SJ, Azimifar SB, Mann M.

Press Release

Nature Biotechnology | NEWS AND VIEWS

The Impact II, a Very High-Resolution Quadrupole Time-of-Flight Instrument (QTOF) for Deep Shotgun Proteomics

Mol Cell Proteomics. 2015 Jul;14(7):2014-29. doi: 10.1074/mcp.M114.047407.

Beck S, Michalski A, Raether O, Lubeck M, Kaspar S, Goedecke N, Baessmann C, Hornburg D, Meier F, Paron I, Kulak NA, Cox J, Mann M

Time- and compartment-resolved proteome profiling of the extracellular niche in lung injury and repair

Molecular Systems Biology, July 6, 2015

H.B. Schiller, I.E. Fernandez, G. Burgstaller, C. Schaab, R.A. Scheltema, T. Schwarzmayr, T.M. Strom, O. Eickelberg and M. Mann [more]

A double-barrel LC-MS/MS system to quantify 96 interactomes per day

Mol Cell Proteomics. 2015 Apr 17.

Hosp F, Scheltema RA, Eberl C, Kulak NA, Keilhauer EC, Mayr K, Mann M. [more]

Widespread Proteome Remodeling and Aggregation in Aging C. elegans

Cell, May 7, 2015 DOI: 10.1016/j.cell.2015.03.032

Walther DM, Kasturi P, Zheng M, Pinkert S, Vecchi G, Ciryam P, Morimoto RI, Dobson CM, Vendruscolo M, Mann M, Hartl FU.

Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links

Science 1 May 2015

Räschle M, Smeenk G, Hansen RK, Temu T, Oka Y, Hein MY, Nagaraj N, Long DT, Walter JC, Hofmann K, Storchova Z, Cox J, Bekker-Jensen S, Mailand N, Mann M

Single muscle fiber proteomics reveals unexpected mitochondrial specialization

EMBO Rep. 2015 Feb 2

Murgia M, Nagaraj N, Deshmukh AS, Zeiler M, Cancellara P, Moretti I, Reggiani C, Schiaffino S, Mann M

Mass spectrometry of HLA-I peptidomes reveals strong effects of protein abundance and turnover on antigen presentation

Mol Cell Proteomics (2015)

Bassani-Sternberg M, Pletscher-Frankild S, Jensen LJ, Mann M

[more]

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Cell-Type-Resolved Quantitative Proteomics of Murine Liver

Cell Metabolism

S. Babak Azimifar, Nagarjuna Nagaraj, Juergen Cox, Matthias Mann

Image: Max Iglesias

Structural Model of a CRISPR RNA-Silencing Complex Reveals the RNA-Target Cleavage Activity in Cmr4

Molecular Cell (2014)

Christian Benda, Judith Ebert, Richard A. Scheltema, Herbert B. Schiller, Marc Baumgärtner, Fabien Bonneau, Matthias Mann, Elena Conti

[more]

The Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High Performance Quadrupole and an Ultra-High Field Orbitrap Analyzer

MCP (2014)

Richard Alexander Scheltema, Jan-Peter Hauschild, Oliver Lange, Daniel Hornburg, Eduard Denisov, Eugen Damoc, Andreas Kuehn, Alexander Makarov and Matthias Mann

[more]

Ultradeep Human Phosphoproteome Reveals a Distinct Regulatory Nature of Tyr and Ser/Thr-Based Signaling

Cell Reports (2014)

Kirti Sharma, Rochelle C.J. D’Souza, Stefka Tyanova, Christoph Schaab, Jacek R. Wisniewski, Juergen Cox and Matthias Mann

[more]

The growing landscape of lysine acetylation links metabolism and cell signalling

Nature Reviews Molecular Cell Biology 15, 536–550 (2014) doi:10.1038/nrm3841

Chunaram Choudhary, Brian T. Weinert, Yuya Nishida, Eric Verdin & Matthias Mann

[more]

Time-resolved dissection of early phosphoproteome and ensuing proteome changes in response to TGF-β

Sci. Signal., 22 July 2014 - Vol. 7, Issue 335, p. rs5 - DOI: 10.1126/scisignal.2004856

J D'Souza RC, Knittle AM, Nagaraj N, van Dinther M, Choudhary C, Ten Dijke P, Mann M, Sharma K.

[more]

Complete proteome analysis and its uses in cell biology and biomedicine

Research talk at the Nobel Foundation, Stockholm, 17 October 2013

We have contributed a chapter to the book CIRCADIAN CLOCKS Handbook of Experimental Pharmacology edited by Martha Merrow and Achim Kramer

We review the proteomics approaches applied to date in the circadian field, and we also discuss the exciting potential of using cutting-edge proteomics technology in circadian biology.

Maria S. Robles and Matthias Mann, Proteomic Approaches in Circadian Biology in Circadian Clocks (Handbook of Experimental Pharmacology) 2013

Science. 2013 Apr 26

Eavesdropping on Immune Cells - Max Planck Scientists Analyze How the Immune System Communicates

Press Release (PDF)

PubMed

The Coming Age of Complete, Accurate, and Ubiquitous Proteomes

Molecular Cell, Volume 49, Issue 4, 583-590, 21 February 2013
Mann M, Kulak N, Nagaraj N, Cox J

High-resolution mass spectrometry (MS)-based proteomics has progressed tremendously over the years. For model organisms like yeast, we can now quantify complete proteomes in just a few hours.

EU Grant Awarded for Dementia Research

Max Planck Researchers receive 13.9 Million Euros in EU funding. The ERC Synergy Grant is the most highly endowed research grant of the European Union and was awarded this year for the first time.[more]

Press Release (PDF)