Sequencing of the whole genome (genomics) permits to unravel the biological capacity of an organism. The sequences of all proteins can be predicted based on the complete DNA sequence, resulting in a "theoretical proteome". This is complemented by experimental analysis of the protein content of an organism (proteomics).
The department is interested in the biology of salt loving organisms, mainly halophilic archaea.
We have sequenced the genome of Halobacterium salinarum strain R1 (DSM 671).
We have sequenced the genome of Natronomonas pharaonis strain Gabara (DSM 2160).
We have sequenced the genome of Haloquadratum walsbyi strain HBSQ001 (DSM 16790).
Cytosolic proteome of Halobacterium
Proteomics has been applied to determe the protein inventory of Halobacterium salinarum. The cytosolic proteome was analyzed by 2D gel electrophoresis in combination with MALDI-TOF MS analysis.
Membrane proteome of Halobacterium
Membrane proteomics was used to analyze the integral membrane proteins of Halobacterium. It consists of 1D gel electrophoresis followed by in-gel trypsin digestion and LC/MS/MS.