The Imaging Facility gives researchers access to sophisticated fluorescence microscopes for the observation and analysis of live or fixed organisms and cells. The scale of samples ranges from whole (small) organisms to single molecules with super-resolution microscopy. We also have high-end flow cytometers, which allow analysis and sorting of cells in many fluorescence channels at high speed. Users get personal training to use equipment and software as needed for their specific experiments, as well as support for data processing and especially quantitative image analysis.
Currently we have five light microscopes:
- WF1: Widefield microscope GE DeltaVision Elite, including laser module for TIRF (Total Internal Reflection) and FRAP (photobleaching, photoswitching)
- WF2: Widefield microscope Leica DMI-6000 with high-sensitivity sCMOS camera
- CF1: Laser-scanning confocal microscope Zeiss LSM780, including a module for FCS (fluorescence correlation spectroscopy) and RICS (Raster Image Correlation Spectroscopy)
- CF2: Laser-scanning confocal microscope Leica SP8, including resonant scanner and module for FLIM (Fluorescence Lifetime Imaging, from PicoQuant)
- SR1: Super-resolution microscope Zeiss Elyra PS.1 with modules for SMLM (single-molecule localisation microscopy; PALM, STORM, PAINT) and SIM (structured illumination microscopy) as well as dual-camera module with image splitter
All systems are equipped with motorised stage, temperature control and CO2 incubation.
For the analysis of cells we offer access to a Thermofisher Attune NxT high-speed flow cytometer, for fluorescence-activated cell sorting (FACS) we have a BD FACSAria III. Both are equipped with excitation lasers ranging from UV (375 and 405 nm) to red (633 nm), and emission filters for practically all fluorophores.