The high-throughput laboratory (H.T.L.) was established in 2005 in the Department of Stefan Jentsch.
In collaboration with personal at the MPIB ,LMU and TU, various high-throughput custom-designed assays were developed and optimized to screen for novel genetic and protein interactions in attempt to identify previously undefined cellular pathways and elucidate new protein functions.
During my tenure in the Jentsch department, my major focus was to provide expertise in assay development towards high-throughput applications and high content screening (HCS), as well genetic interactions and protein domain mapping. To this end, H.T.L. provides state-of-the-art equipment for cell-based screening platforms such as robotic liquid handling stations, plate readers, automated microscopes and peptide array synthesis. The services primarily includes developing novel high-throughput assays as well as existing bench protocols to an automated robotic system.
- Automated liquid handling (contact and free dispense)
- Synthetic genetic array (SGA)
- Automated confocal microscopy
- Fixed-cell assays
- Live-cell assays
- Protein-protein interactions (Y2H, SplitVinus, DHFR)
- High-throughput phenotyping
I have extensive experience primarily preforming yeast screens with various genomic libraries including yeast knock-out, GFP and temperature sensitive collections. Assay and technology development for high-throughput screening (lab automation, automated microscopy, image analysis, data management), robotic liquid handling processes (reformatting & replication of libraries, normalisation of products to identical concentrations, cherry/hit picking, preparation of dilution series, PCR-setup)
My research and development interests include the following:
- Application of automated image-based screening at subcellular resolution.
- Analysis of kinetic parameters of biological processes in medium-throughput mode with live cell automated microscopy.
- Identification of new novel gene functions, interactions and phenotype after depletion, knock-down or over-expression.
- Development of image analysis solutions for imaging projects in low and high throughput microscopy (CellProfiler, Harmony, MatLab, KNIME, Cytoscape).
- Liquid handling for all sorts of applications.
Do it yourself:
After an introduction to the various robotic platforms followed by a complete
training program , the H.T.L. provides individuals with the opportunity to
perform automated experiments or projects on their own with the instruments
provided in the Laboratory.