EMBL scripts for high-accuracy CLEM

This website provides access to the scripts for hich-accuracy correlated light and electron micrscopy as described in these publications

Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M., Briggs, J.A.G., 2011. Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. J. Cell Biol. 192, 111–119. doi:10.1083/jcb.201009037
Kukulski, W., Schorb, M., Welsch, S., Picco, A., Kaksonen, M., Briggs, J.A.G., 2012. Precise, correlated fluorescence microscopy and electron tomography of lowicryl sections using fluorescent fiducial markers. Methods Cell Biol. 111, 235–257. doi:10.1016/B978-0-12-416026-2.00013-3
Schorb, M., Briggs, J.A.G., 2014. Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity. Ultramicroscopy 143, 24–32. doi:10.1016/j.ultramic.2013.10.015
Schorb M., Gaechter L., Avinoam O., Sieckmann F., Clarke M., Bebeacua C., Bykov Y., Sonnen A. F-P., Lihl R., Briggs J.A.G., 2016. New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography. J. Struct. Biol., doi:10.1016/j.jsb.2016.06.020

This software is available under the GPL v.3 for academic and non-commercial use.

If you are interested in using the scripts or parts thereof for a commercial application, please contact us.