Proteomics/Mass Spectrometry

Proteomics/Mass Spectrometry

The Mass Spectrometry Core Facility uses state-of the-art chromatography systems, mass spectrometers and workflows for the in-depth analysis of biomolecules. Our services include:

  • Identification of proteins (in-gel or in-solution)
  • In-depth proteome analysis (with and without fractionation)
  • Interaction proteomics by Affinity Purification Mass Spectrometry (AP-MS)
  • Relative quantification of peptides and proteins (label-free quantification, SILAC, dimethyl or TMT labelling)
  • Post-translational modification identification (e.g. phosphorylation, ubiquitination, acetylation or glycosylation). Low stoichiometry modifications can be brought into view by enrichment (e.g. Fe-IMAC for phosphorylation)
  • Crosslinking-MS (XL-MS) to extract protein structure distance information
  • Hydrogen-deuterium exchange (HDX-MS) to uncover interaction interfaces
  • Intact mass of proteins and other biomolecules (Topdown-MS)
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