Proteomics Mapping
Members: Matthias Mann, Nagarjuna Nagaraj, Jacek R. Wiśniewski
Qualitative and quantitative mapping of proteomes is a key goal of proteomics. Due to complexity of the eukaryotic proteomes, a wide scale identification of proteins, their structural variants, and posttranslational modifications require a technology comprising selected methods for processing of biological material and mass spectrometric analysis, informatics and bioinformatics tools. Each of these elements as well as their mutual tuning is essential for achievement of satisfactory results.
Processing of biological samples, their fractionation, and generation of samples for mass spectrometric analysis are the most complex and critical steps of a protein mapping experiment. Therefore, a preparative strategy has to be well selected considering the properties of the analyzed sample, the nature of the proteins of interest, and expected dynamic range. Moreover, the composition of the final product of the biochemical work should be carefully defined to enable efficient identification of proteins by mass spectrometric analysis.
The activities of this group are focusing on mapping of proteomes and subproteomes using the established protocols as well as continuous development of methods for tissue-processing and design of novel fractionation strategies. The mapping efforts provide insights in the composition of various proteomes including body fluids and tissues proteomes. The novel fractionation strategies should extend the proteome coverage for highly charged, very large, and membrane proteins under the prerequisite that the number of generated fractions is kept as low as possible.
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