In vivo proteomics
Members: Matthias Mann, Charo Robles
Our group aims at better understanding the molecular mechanisms regulating biological processes in physiology (i.e. circadian mechanisms; vessel formation) and pathology (i.e. infection, tumor).
To achieve this goal, besides performing experiments with cell lines, we use primary cells since they are neither transformed nor immortalized and therefore more representative of cells in vivo. We use the mouse as an animal model for in vivo studies.
We mainly apply SILAC in combination with high resolution analysis on LTQ-Orbitrap to perform quantitative studies at the proteome and phosphoproteome level. Our lab has extended the SILAC technique to primary cell culture and moreover to mice. Recently, we have generated the first colony of mice labeled with 13C6-substituted version of lysine (Kruger et al. (Cell 2008, 134(2), 353-364)), which can be used to analyze quantitatively proteomes in vivo.
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