Nature Reviews Molecular Cell Biology 7, 952-958 (December 2006) | doi:10.1038/nrm2067  

Functional and quantitative proteomics using SILAC Matthias Mann1  About the author

Matthias Mann1

1Department of Proteomics and Signal Transduction, Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany

Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and — as a quantitative phosphoproteomics technology — directly uncovers important points in the signalling pathways that control cellular decisions.

 

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