Super-SILAC mix for quantitative proteomics of human tumor tissue.

Geiger T, Cox J, Ostasiewicz P, Wisniewski JR, Mann M.

Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.

We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.

http://www.ncbi.nlm.nih.gov/pubmed/