Universal sample preparation method for proteome analysis

Jacek R Wiśniewski, Alexandre Zougman, Nagarjuna Nagaraj & Matthias Mann

Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.

We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

http://www.nature.com/nmeth/journal/vaop/ncurrent/abs/nmeth.1322.html