Native gel electrophoresis and SDS-PAGE according to Laemmli or Schaegger are established for the separation of proteins ranging from the highest to the lowest molecular weights. 

Analytical or preparative scale isoelectric focusing is available using IPG-strips or Free-Flow-Electrophoresis (ProMetHEUS, FFE Weber). Offgel Electrophoresis (Agilent) can be used to prefractionate proteins in solution according to their isoelectric point.

High resolution 2D-PAGE using wide-ranging pH gradients (pH 3-10) or very narrow gradients (1 pH unit, e.g. pH 6-7) in the first dimension and any kind of SDS gel system in the 2nd dimension can be applied. For multiplexing, based on 2D-gel electrophoresis DIGE (GE Healthcare) can be used.

All kind of staining methods (Coomassie, silver,Zn negativ, fluorescent, immunostain) are established. 

Gels can be digitalized, quantitatively analyzed and matched (Samespots,Nonlinear Dynamics). Spots can be excised by an automatic spotpicker (Exquest, Biorad).

 

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© 2011, Max Planck Institute of Biochemistry, Martinsried