Despite mass spectrometry covers a broad spectrum of methods for the characterisation and identification of proteins, Edman sequencing is still an appropriate method for several applications.

N-terminal sequencing of intact proteins

Identification of cleavage sites

Sequence information about proteins of species without genome information

We utilize an Applied Biosystems Model 492 Procise and a Model 492 Procise cLC protein/peptide sequencer with an on-line Applied Biosystems Model 140C PTH Amino Acid Analyzer.

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For detailed information about Edman sequencing and method description read: "Edman sequencing".

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© 2011, Max Planck Institute of Biochemistry, Martinsried