All kind of chromatography technologies are established to reduce the enormous complexity present in proteomes.

Reversed Phase (RP)-HPLC from nano- to semi-preparative scale (0.075 mm - 4.6 mm i.d.) (Agilent 1100 series).

Multidimensional (MD)-chromatography, using chromatofocussing chromatography followed online by RP-HPLC, to separate proteins according to their isoelectric point and hydrophobic interactions (Beckman PF2D) .

Multidimensional (MD)-chromatography, using ion exchange followed online by RP-HPLC, e.g. for serum analysis or separation of highly complex peptide mixtures (Ultimate 3000, Ultimate, Dionex).

Ion exchange chromatography for protein and peptide separation according to net charge (SMART, Amersham Biosciences).

Size exclusion chromatography for protein separation according to protein size.

Affinity chromatography, e.g. IMAC (immobilised metal chelate chromatography) to enrich phosphopeptides.

Immuno affinity chromatography, e.g. to deplete serum from albumin and other high abundant proteins.

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© 2011, Max Planck Institute of Biochemistry, Martinsried