A Mammalian Organelle Map
Cell, Vol 125, 187-199, 7 April 2006
Leonard J. Foster,1,2 Carmen L. de Hoog,1,2 Yanling Zhang,3,4 Yong Zhang,3,4 Xiaohui Xie,5 Vamsi K. Mootha,5,6 and Matthias Mann1,3,
1 Center for Experimental BioInformatics (CEBI), Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
2 Centre for Proteomics, Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
3 Department of Proteomics and Signal Transduction, Max-Planck Institute for Biochemistry, Martinsried, Germany D-82152
4 Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China
5 Broad Institute of Harvard and MIT, Cambridge, MA 02139, USA
6 Department of Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USA
Protein localization to membrane-enclosed organelles is a central feature of cellular organization. Using protein correlation profiling, we have mapped 1,404 proteins to ten subcellular locations in mouse liver, and these correspond with enzymatic assays, marker protein profiles, and confocal microscopy. These localizations allowed assessment of the specificity in published organellar proteomic inventories and demonstrate multiple locations for 39% of all organellar proteins. Integration of proteomic and genomic data enabled us to identify networks of coexpressed genes, cis-regulatory motifs, and putative transcriptional regulators involved in organelle biogenesis. Our analysis ties biochemistry, cell biology, and genomics into a common framework for organelle analysis.
http://www.cell.com/content/article/abstract?uid=PIIS0092867406003692
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