Proteomics and Signal Transduction

Mass spectrometry	SILAC	Bioinformatics	Complete proteome
Clinical proteomics	FASP	PTM	Interaction proteomics

Group

About us
-Overview for non-scientists-

Über uns
-Einführung für Interessierte-

Director

Prof. Dr. Matthias Mann

MaxQuant

Cox J, Mann M., Nat. Biotech. 26, 1367 - 1372.
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. more...

Cox J et al., Nat Protoc. 2009;4(5):698-705.
A practical guide to the MaxQuant computational platform for SILAC-based
quantitative proteomics. more...

News & Selected Publications

Matthias Mann awarded 2012 Jeantet Prize for Medicine

Matthias Mann has been awarded the 2012 Louis-Jeantet Prize for medicine for his work on developments in mass spectrometry that have revolutionised the analysis of proteins and of their functions. [Press Release Louis-Jeantet Foundation (PDF)]

Louis-Jeantet Prize Winner 2012: Perspective

Proteomics for biomedicine: a half-completed journey

25 Jan 2012 EMBO Molecular Medicine.
Matthias Mann

[article]

Matthias Mann awarded 2012 Ernst Schering Prize

The Schering Foundation, Berlin, honors the biochemist for the development of novel methods for the analysis of proteins.
[Press Release Schering Foundation (PDF)]

Matthias Mann awarded 2012 Leibniz Prize

In recognition of new methods of protein analysis
[Press Release German Research Foundation]

Mann group most highly cited in protein research.

According to an ISI analysis performed by LabTimes, the Mann group has the most citations of researchers working in protein science related areas. For the years of 1998-2009. Mann’s group topped the list at 37,178 citations for 287 papers. See pdf here.

Quantitative, High-Resolution Proteomics for Data-Driven Systems Biology.

Annu Rev Biochem. May 2011.
Cox J, Mann M. - www.annualreviews.org

[abstract] [pubmed]

John bennett fenn, 1917-2010

J Am Soc Mass Spectrom. 2011 Mar;22(3):602-3.
Mann M.

Matthias Mann on the First Quantitative & Proteome-Wide Study of Lysine Acetylation

Article Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.

Precision Mapping of an In Vivo N-Glycoproteome Reveals Rigid Topological and Sequence Constraints.

Cell, Volume 141, Issue 5, 897-907, 28 May 2010
D. Zielinska, F. Gnad, J. Wisniewski, M. Mann - www.cell.com -

[abstract] [phosida]

Decoding signalling networks by mass spectrometry-based proteomics.

Nature Reviews Molecular Cell Biology 11, 427-439 (June 2010)
Chunaram Choudhary and Matthias Mann - www.nature.com -

[abstract]

Super-SILAC mix for quantitative proteomics of human tumor tissue.

Nat Methods. 2010 Apr 4.

Geiger T, Cox J, Ostasiewicz P, Wisniewski JR, Mann M.
- www.nature.com -

[abstract] [Recommended by Faculty of 1000 Biology]

Quantitative Proteomics Reveals Subset-Specific Viral Recognition in Dendritic Cells.

Immunity, Volume 32, Issue 2, 279-289, 18 February 2010

Luber CA, Cox J, Lauterbach H, Fancke B, Selbach M, Tschopp J, Akira S, Wiegand M, Hochrein H, O'Keeffe M, Mann M. - www.cell.com -

[abstract]

Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

Sci. Signal., 12 January 2010
Vol. 3, Issue 104, p. ra3

Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M. - www.sciencemag.org -

[abstract]

Quantitative proteomic analysis of single pancreatic islets.

Waanders LF, Chwalek K, Monetti M, Kumar C, Lammert E, Mann M.,
Proc Natl Acad Sci U S A. 2009 Oct 21. - www.pnas.org

[abstract]

Lysine acetylation targets protein complexes and co-regulates major cellular functions.

Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.,Science 14 August 2009: 834-840. - www.sciencemag.org -

[abstract]

BREAKTHROUGH OF THE YEAR:The Runners-Up

Science news stories - www.sciencemag.org -
Sequencing and quantitation of the first entire yeast proteome was selected by Science as one of the 10 Breakthroughs of the year. (See the paper here and see item 5 in "Watching proteins at work"). more...

Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast

L. M. F. de Godoy et al., Nature 455, 1251-1254 (2008)

Mass spectrometry is a powerful technology for the analysis of large numbers of endogenous proteins. However, the analytical challenges associated with comprehensive identification and relative quantification of cellular proteomes have so far appeared to be insurmountable. Here, using advances in computational proteomics, instrument performance and sample preparation strategies, we compare protein levels of essentially all endogenous proteins in haploid yeast cells to their diploid counterparts. more...

MaxQuant Summerschool 2012

the FASP procedure

Wisniewski J.R. et al.
Nat. Methods 6:359-362.

Filter Aided Sample Preparation (FASP) method allows proteomic analysis of SDS-lysates with essentially any class of proteins and origin of biological material. In particular, FASP enables digestion of membrane proteins under conditions previously applied only to soluble proteins.
more...