The Imaging Facility gives researchers access to sophisticated fluorescence microscopes for the observation and analysis of live or fixed organisms and cells. The samples range from whole (small) organisms to single molecules with super-resolution microscopy. Users get personal training to use equipment and software as needed for their specific experiments. We also have a top-level flow cytometer, which allows analysis and sorting of cells in many fluorescence channels at high speed.
Currently we have five light microscopes:
- CF1: Laser-scanning confocal microscope Zeiss LSM780, including module for FCS (fluorescence correlation spectroscopy) and RICS (Raster Image Correlation Spectroscopy)
- CF2: Laser-scanning confocal microscope Leica SP8, including resonant scanner and module for FLIM (Fluorescence Lifetime Imaging, from PicoQuant)
- CF3: Spinning-disk confocal microscope Perkin Elmer UltraVIEW Vox with EMCCD camera, including laser module for FRAP (photobleaching, photoswitching)
- WF1: Widefiel microscope GE DeltaVision Elite, including laser module for TIRF (Total Internal Reflection) and FRAP (photobleaching, photoswitching)
- SR1: Super-resolution microscope Zeiss Elyra PS.1 with modules for SMLM (single-molecule localisation microscopy; PALM, STORM, PAINT) and SIM (structured illumination microscopy) as well as dual-camera module with image splitter
All systems are equipped with motorised stage, temperature control and CO2 incubation.
Fort analysis and sorting of cells at high speed we have a BD FACSAria III high-speed flow cytometer.
- Excitation lasers:
- Sorting options:
- 5ml FACS tubes (2-way/4-way, cooled)
- 15ml Falcon tubes (2-way, cooled)
- 96-well plates (cooled)
- microscope slides
- Eppendorf tubes (2-way/4-way, cooled)