EU-Projects at the MPIB

 

ERC Advanced Grant: EXORICO

(01.10.2017 – 30.09.2022)

Elena Conti (MPI Dept.: Structural Cell Biology)

Full title: “EXORICO - Exosome and ribosome coupling“

Budget: 2 Mio Euro

To date, mechanistic studies on the macromolecular complexes that synthesize or degrade RNAs or proteins have investigated these machines individually to understand how they execute different steps in the gene expression process. Although the individual complexes catalyze their reactions independently of each other in vitro, increasing evidence suggests that they function in a highly coordinated manner in vivo. The molecular basis for such a coordination remains largely unknown. Here, we will take these analyses to the next level and visualize how a major RNA degradation machine, the exosome, is directly coupled to the protein-synthesis machine, the ribosome. In particular, we want to study two different exosome-ribosome assemblies that underpin opposite outcomes of RNA degradation: a constructive function of the nuclear exosome in the maturation of the large ribosomal subunit and a destructive function of the cytoplasmic exosome in the elimination of ribosome-bound mRNAs. Overall, this work will provide important insight into the principles that coordinate different steps of eukaryotic gene expression.


MSCA-IF: POLAR

(01.09.2017 – 31.08.2019)

Leon Harrington (MPI Dept.: Cellular and Molecular Biophysics)

Full title: “POLAR – Synthetic Mimicry of Cellular Polarisation”

Biological self-organisation through spontaneous symmetry breaking is essential for the emergence and maintenance of life. Cell polarity, where an asymmetric distribution of molecules is achieved within a cellular compartment, is one of the simplest and most studied examples, but still there are big lacks of knowledge. A synthetic system capable of mimicking cellular polarization in an artificial compartment is planned to be built and thus more insight in this process should be gained.


ERC Consolidator Grant: FocAd

(01.07.2017 – 30.06.2022)

Naoko Mizuno (MPI Group: Cellular and Membrane Trafficking)

Full title: “FocAD – Structural Studies on Focal Adhesions"

Budget: 2 Mio Euro

Focal adhesions (FA) are gigantic protein complexes whose formation is triggered by integrin ligand activation. FAs represent (1) a sensing platform and anchoring points to the outside environment and, (2) a machinery to transduce this information by initiating signaling pathways for cell growth, survival, migration and polarization. Failure in the regulation of FA leads to developmental defects and contributes to the formation of aggressive cancers. Hence, understanding FA formation is the basis for counteracting these detrimental effects


MSCA-IF: REVERSING TAUOPATHY

(01.06.2017 – 31.05.2019)

Saurabh Gautam (MPI Dept.:Cellular Biochemistry)

Full title: “REVERSING Tauopathy - Identifying and deciphering the mechanism of the cellular machinery responsible for disaggregation of intracellular prion-like Tau aggregates"

Tauopathies are a group of neurodegenerative diseases, the most common of them being Alzheimer’s disease, which are associated with the aggregation of protein Tau in the brain. Therapeutics to cure these tauopathies are urgently needed, but the mechanism of Tau aggregation and its pathogenesis giving rise to neurodegenerative diseases is not yet understood. We aim to identify the conditions, the cellular machinery responsible and the mechanism of disaggregation of intracellular prion-like Tau aggregates in cell culture.


MSCA-IF: Immuno RQC

(01.05.2017 – 30.04.2019)

Deborá Broch Trentini Schmidt (MPI Dept.:Cellular Biochemistry)

Full title:“Immuno-RQC - Role of Listerin ubiquitin ligase in MHC-I antigen presentation"

Mammalian cells present a fingerprint of their proteome through the display of endogenous peptides on MHC-I complexes. This allows for CD8+ T cells to recognize and kill cells infected with viruses or other intracellular parasites, or cells accumulating aberrant proteins resulting from malignant transformation. Here we test whether the E3 ubiquitin ligase Listerin, which marks nascent polypeptides resulting from defective mRNAs for degradation, also contributes to the production of peptides for MHC-I loading. Our major aim is to investigate a potential involvement of Listerin in antigen presentation.


MSCA-IF: Cytokineproteomics

(01.03.2017 – 28.02.2019)

Maria Tanzer (MPI Dept.: Proteomics and Signal Transduction)

Full title:“Cytokineproteomics – Investigating inflammatory signaling by combining phospho- and ubiquitin proteomics with CRISPR/Cas9 technology”

Deregulation of pro-inflammatory cytokine signalling is the underlying cause for many inflammatory diseases. Thus, understanding the molecular mechanism of cytokine signalling is necessary to develop suitable therapies. This study will investigate the mechanisms and the relationship between phosphorylation and ubiquitylation in cytokine signalling in an unprecedented way and has the potential to result in the identification of new therapeutic strategies for the treatment of a range of inflammatory diseases.


MSCA-ITN-ETN: TEMPERA

(01.03.2017 – 28.02.2021)

MPI Group: Jürgen Cox

Coordinator: Enrico Cappellini, UCPH

6 partners (+4 external partners) in 5 countries

Budget: total: 2.2 Mio Euro; MPI: 250.000 Euro

Full title:“TEMPERA: Teaching Emerging Methods in Palaeoproteomics for the European Research Area”

TEMPERA will provide international, and intersectoral training for early stage researchers (ESRs) to read ancient protein sequences and interpret the recovered information. This will improve knowledge about production techniques and chemical preservation of cultural heritage materials, ultimately improving their safeguard and conservation. TEMPERA will also guide the ESRs to develop the advanced interdisciplinary knowledge required to achieve seamless integration of this innovative analytical approach with the established principles and practices of cultural heritage restoration and conservation.

MSCA-IF: Minimal-phagocyte

(01.09.2016 – 31.08.2018)

Kristina Ganzinger (MPI Dept.: Cellular and Molecular Biophysics)

Full title: “Minimal-phagocyte: Reconstitution of the basic molecular mechanism of phagocytes – a bottom-up synthetic biology approach”

The ability of cells to engulf large objects, such as invading microorganisms or apoptotic cells is crucial to innate immunity and tissue remodelling. The molecular basis of this process – phagocytosis – is complex, involving numerous receptors and signaling pathways. Although the key molecular players in individual phagocytic pathways have been identified, we still know very little about the basic biophysics of phagocytosis. The aim of this project is to fill this gap in our knowledge.


MSCA-ITN-ETN: EDGE

(01.05.2016 – 30.04.2020)

MPI Group: Andreas Pichlmair

Coordinator: Søren Riis Paludan,  AARHUS UNIVERSITET

9 partners in 6 countries

Budget: total: 4 Mio Euro; MPI: 250.000 Euro

Full title: “EDGE: Training network providing cutting-EDGE knowlEDGE on Herpes Virology and Immunology”

Key research aims of EDGE are to improve the basic understanding of the interactions between herpesviruses and host cells and to uncover implications for the clinical outcome of infections as well as development of vaccines. EDGE will provide a comprehensive and cross-disciplinary structured curriculum for doctoral students in the fields of virology and immunology.


FETOPEN: MSmed (co-financed by the European Commission)

(01.12.2015 – 30.11.2019)

MPI Group:Jürgen Cox

4 partners in 3 countries

Budget: total: 3.6 Mio Euro; MPI: 699.000 Euro

Full Title: “Mass spectrometric technology for next generation proteomics in systems medicine”

The project is expected to establish mass spectrometry based proteomics in systems medicine, making all workflows and mass spectrometry platforms available to the community. These workflows will be used as the basis of myriad applications in biomedicine, even in the clinic. This in turn will lead to a new eco-system around improved diagnosis, elucidations of disease mechanisms and drug action.


ERA-NET: ERASE (financed by the European Commission and the BMBF)

(01.05.2015 – 30.04.2018)

MPI Group: Andreas Pichlmair

6 partners in 4 countries

Budget: total: 2 Mio Euro; MPI: 388.000 Euro

Infect-ERA:"ERASE - Evaluating RNA/DNA bound proteins across species."

The multinational team aims to identify conserved defense mechanisms against viruses. Hereby, the researchers hope to discover candidate mechanisms that can be used for future antiviral therapeutic approaches.


ERC Starting Grant: GAMES

(01.06.2015 – 31.05.2020)

MPI Group: Gurumoorthy Krishnamoorthy

Budget: 1,5 Mio Euro

Gut Microbiota in Nervous System Autoimmunity: Molecular Mechanisms of Disease Initiation and Regulation.


ERC Starting Grant: Autophagy in vitro

(01.04.2015 – 31.03.2020)

MPI Group: Thomas Wollert

Budget: 1,5 Mio Euro

Reconstituting Autophagosome Biogenesis in vitro.


ERC Advanced Grant: SUMOGROUP

(01.02.2014 – 31.01.2019)

MPI Group:  Stefan Jentsch

Budget: 2,5 Mio Euro

Posttranslational modification (PTM) of proteins by ubiquitin family proteins is of fundamental importance for cellular function, regulation and development. The ubiquitin-related protein SUMO frequently targets protein groups rather than individual protein substrates. SUMOGROUP addresses the function of protein group SUMOylation and expects to break new ground in areas of cellular regulation and cell biology in general.



Marie Curie Initial Training Network: PloidyNet

(01.10.2013 - 30.09.2017)

Coordinator: René Medema (NKI, Amsterdam)

Manager:     Henri van Luenen (NKI, Amsterdam)

MPI Group: Zuzana Storchova

11 Partners in 4 countries, Budget: 3 Mio Euro.

Aneuploidy, an abnormal number of chromosomes, is a hallmark of cancer cells and affects the majority of all human tumours. PloidyNet aims to determine and compare the molecular consequences of different levels of aneuploidy, both in vivo and in vitro. At the same time the network will train young researchers to become experts in the field of aneuploidy.



ERC Synergy Grant: ToPAG

(01.06.2013 – 31.05.2019)

MPIB Groups: Franz-Ulrich HartlWolfgang Baumeister, Matthias Mann

MPIN Group:  Rüdiger Klein

Budget: 14 Mio Euro.

Formation of amyloid-like protein aggregates is the hallmark of a number of neurodegenerative diseases, but how the aggregation process is linked with cytotoxicity and cell death remains unclear. The goal of ToPAG is to elucidate the basic mechanisms of aggregate toxicity and how it affects the biological system in its entirety. Understanding aggregation toxicity will be invaluable in developing novel therapeutic strategies for some of the most debilitating diseases of our time.

MSCA-CIG: SORTMECH

(01.04.2013 – 31.03.2017)

Julia von Blume (MPI Research Group.: Molecular Basis of Protein Trafficking)

Full title: “SORTMECH – Characterization of a novel sorting pathway at the Trans Golgi Network (TGN)”

Budget: 100.000 Euro

The Trans Golgi Network (TGN) is the central sorting station for newly synthesised proteins in the cell. Similar to a postal distribution center, incoming cargo is selected, sorted, and packaged to be carried to its final cellular destination by the postman in charge. Due to the involvement of numerous different cargoes and carriers for the different destinations this process of protein sorting at the TGN is highly sophisticated. We will characterize a new sorting mechanism in detail to identify components of the sorting machinery on both cytosolic and luminal faces of the TGN.



ERC Advanced Grant: SIGNAL2THEHUB

(01.03.2013 – 28.02.2018)

MPI Group:  Reinhard Fässler

Budget: 2,5 Mio Euro.

Integrin-mediated cell adhesion is essential for the development and homeostasis of multicellular organisms. Integrins recruit adhesion proteins into large signalling hubs called adhesomes. SIGNAL2THEHUB investigates the mechanistic contribution of individual integrin classes to the recruitment of proteins to the adhesome and how adhesome signalling is induced.


ERC Starting Grant: Flight Muscle

(01.02.2013 – 31.01.2018)

MPI Group: Frank Schnorrer

Budget: 1,5 Mio Euro

Mechanistic dissection of myofibrillogenesis and sarcomerogenesis in insect muscle.


ERC Starting Grant: iViP

(01.11.2012 – 31.10.2017)

MPI Group: Andreas Pichlmair

Budget: 1,5 Mio Euro.

Impact of virus infection on the host proteome.


ERC Starting Grant: CiliTransport

(01.09.2012 – 31.08.2017)

MPI Group: Esben Lorentzen

Budget: 1,5 Mio Euro.

Structural Studies and Regulation of Intraflagellar Transport Complexes - The cilium is an organelle that protrudes from the cell body and is responsible for the motility of unicellular organisms and of vertebrate cell types such as sperm cells. In addition, most vertebrate cells have primary non-motile cilia important for sensory reception and signalling. The assembly and function of cilia rely on intraflagellar transport (IFT), the bi-directional movement of macromolecules between the cell body and the cilium.


ERC Advanced Grant: DEAD2THEEND

(01.04.2012 – 31.03.2017)

MPI-Group: Elena Conti

Budget: 2,5 Mio Euro.

RNA poly(A) tail: the beginning of the endIn this project, we will reconstitute the key protein complexes in mRNA decay from recombinant proteins in vitro. Specifically, we will focus on the evolutionary conserved deadenylation, decapping and exosome-Ski complexes. The reconstituted complexes will be used for structural studies to derive atomic models of the holoenzymes using a combination of X-ray crystallography and cryoelectron microscopy. In parallel to obtaining static views of the individual steps in the pathway, we will establish the assays to study how information from one processing step is passed on to the next in a dynamic manner. We will address the basis for the timing and interrelationship of the conserved enzymatic machineries and the influence of the mRNP composition on their activity. Our final goal is to recapitulate the complex behaviour of the mRNA decay pathway in vitro.



Collaborative Project: 4DCellFate

(01.12.2011 - 30.11.2016)

Coordinator: Luciano di Croce (CRG, Barcelona)

Manager: Mariana Morlans (CRG, Barcelona)

MPI Group: Juerg Mueller

12 Partners in 8 countries, budget: 12 Mio Euro.

The goal of 4DCellFate is to understand how the PRC/NuRD complexes and their plethora of interactions (protein/protein, protein/nucleosome, protein/nucleic acids) regulate cell fate. The ultimate outcome will be to lay the foundations for understanding the role of the PRC/NuRD complexes in ES cell differentiation and cancer, specifically in leukaemogenesis.

Collaborative Project: Euro-MOTOR

(01.02.2011-31.01.2016)

Coordinator: L. van den Berg, UMC Utrecht

Manager: P. Berk, UMC Utrecht

MPI Group: M.Mann

15 Partners (16 Labs) in 7 countries, budget: 11,8 Mio Euro.

Major objective is a better understanding and treatment of the neurotropic degenerative disease Amyotrophic Lateral Sclerosis. Euro-MOTOR aims to detect key genetic drivers of disease susceptibility/progression and to generate a model of disease which can be validated in patients to pave the way for novel therapeutic interventions for this disabling and fatal disease.

ERC Starting Grant: DiseNtAngle

(01.11.2010 – 31.10.2015)

MPI Group: Stephan Gruber

Budget: 1,4 Mio Euro.

Untangling the Bacterial Chromosome: Condensin's Role in Sister Chromosome Separation and its Mechanisms.


FP7 Marie Curie Initial Training Network: RNPnet

(01.11.2011 - 31.10.2015)

Coordinator: Frédéric Allain (ETH, Zürich)

Manager: Regina Notz (ETH, Zürich)

MPI Group: Elena Conti

14 Partners (15 Labs) in 8 countries, budget: 4,2 Mio Euro.

The goal of this ITN (RNPnet) is first to bring together existing labs from different discipline, to join forces and tackle key questions in the field, and second, to produce highly-trained young researchers that will be sensitized to RNA and possess a multidisciplinary approach to research.

Collaborative Project & CSA: Prime-XS

(01.02.2011- 31.01.2015)

Coordinator: Albert Heck, Utrecht University

Manager: Reinout Raijmakers, Utrecht University

MPI Group: M.Mann

12 Partners (15 Labs) in 9 countries, budget: 9,8 Mio Euro.

Major objective: to provide state-of-the-art proteomics technologies to the European biological and biomedical research community. The leading proteomic labs forming Prime-XS will provide access to their facilities, develop new technologies, and will disseminate knowledge and expertise through meetings and training events.

EU FP7 Coordination Action: CommHERE

(01.10.2011 - 30.09.2014)

Coordinator: Ulla Bredberg (Karolinska Institutet, Stockholm)

Manager: Jennie Idegren (Karolinska Institutet, Stockholm)

MPI Group: Anne Katrin Werenskiold

10 Partners in 7 countries, budget: 2 Mio Euro.

The overall aim of CommHERE is to improve communication on the outcome of EU funded health research projects to the media, the general public and other target groups including the EC in all of Europe.

EU FP7 Marie Curie Initial Training Network: Nucleosome4D

(01.11.2009 - 31.10.2013)

Coordinator: Andreas Ladurner, EMBL Heidelberg (LMU, Munich from March 1, 2011 onwards)

Manager: Marzia Sidri, EMBL Heidelberg (Corey Laverty, LMU Munich from March 1, 2011 onwards)

MPI Group: Juerg Müller

16 Partners (21 Labs) in 11 countries, budget: 4,6 Mio Euro.

The main objective of the Network is to provide young researchers with world-class research & training in chromatin biology. Complementary approaches in structural biology, biophysics, cell biology, live-cell imaging, biochemistry, genetics, genomics and bioinformatics will be used in collaborative projects to determine how nucleosomes are remodeled during transcription, when genes are silenced, as cell divide, as stem cells differentiate, during organismal development and in human disease.

ESFRI-Project: Instruct

(Preparatory Phase: 01.04.2008-31.03.2011; Operational Phase: since 01.04.2011)

Coordinator: D. Stuart, University of Oxford

National Coordination: W. Baumeister, MPI Biochemistry

Preparatory Phase: 12 partners in 5 countries, budget. 4.5 Mio Euro. Major objectives: to set up a framework consisting of distributed centres (during preparatory phase 7 core centres), each of which will maintain a set of core technologies such as protein production, NMR, crystallography and different forms of microscopy, including electron microscopy and combine this with a specific biological focus that will drive the development of technological and methodological expertise, notably for the analysis of functional complexes.

Operational Phase: Currently 20 partners in 8 countries, 8 core centres and 7 associate centres. Major objectives: to provide European researchers from academia and industry with access to state-of-the-art structural biology technologies, scientific expertise and training and, by driving innovation at the boundaries between these technologies, stimulate and facilitate research that integrates an understanding of biological structure with cellular function.

Collaborative Project: Prospects

(1.4.2008-31.3.2013)

Coordinator: M.Mann, MPI Biochemistry

Manager: Anne Katrin Werenskiold, MPI Biochemistry

MPI Groups: Mann, Hartl, Baumeister

10 Partners (12 Labs) in 7 countries, budget. 11,7 Mio Euro. Major objectives include the development of much more powerful instrumentation and novel proteomics methods. The goal is to annotate quantitatively the human proteome with respect to protein localization and dynamics.

Integrated Project: Interaction Proteome

(1.1.2004-30.6.2009)

Coordinator: F-U. Hartl, MPI Biochemistry

Manager: Anne Katrin Werenskiold, MPI Biochemistry

11 Partners (14 Labs) in 7 countries, budget. 12 Mio Euro. Major objectives include the establishment of a broadly applicable platform of routine methods for the analysis of protein interaction networks.


Network of Excellence: 3D-EM

(1.3.2004-31.08.2009)

Coordinator: A. Engel, Biozentrum, Basel

Scientific Manager: Anne Katrin Werenskiold, MPI Biochemistry

16 Partners (18 Labs) in 7 countries, budget. 10 Mio Euro. Major objectives include the establishment of synergies between different technologies in structural research and of an extensive training programme in all fields related to structural biology

 
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