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MPI für Biochemie  

Membrane Biochemistry
Dieter Oesterhelt

Genomics and Proteomics of Halophilic Archaea

 

Sequencing of the whole genome (genomics) permits to unravel the biological capacity of an organism. The sequences of all proteins can be predicted based on the complete DNA sequence, resulting in a "theoretical proteome". This is complemented by experimental analysis of the protein content of an organism (proteomics).

Halophilism

The department is interested in the biology of salt loving organisms, mainly halophilic archaea.

Halobacterium salinarum

Halobacterium_Saline

We have sequenced the genome of Halobacterium salinarum strain R1 (DSM 671).

Natronomonas pharaonis

nitrogen metabolism of Natronomonas

We have sequenced the genome of Natronomonas pharaonis strain Gabara (DSM 2160).

Haloquadratum walsbyi

large Haloquadratum cell (journal cover)

We have sequenced the genome of Haloquadratum walsbyi strain HBSQ001 (DSM 16790).

Cytosolic proteome of Halobacterium

2D gel image

Proteomics has been applied to determe the protein inventory of Halobacterium salinarum. The cytosolic proteome was analyzed by 2D gel electrophoresis in combination with MALDI-TOF MS analysis.

Membrane proteome of Halobacterium

1D gel and proteomic processing

Membrane proteomics was used to analyze the integral membrane proteins of Halobacterium. It consists of 1D gel electrophoresis followed by in-gel trypsin digestion and LC/MS/MS.