B. Eversmann, A. Lambacher, T. Gerling, A. Kunze, P. Fromherz, R. Thewes
A neural tissue interfacing chip for in-vitro applications is presented based on an extended 0.5µm, 5 V, standard CMOS process. The chip provides 256 x 128 bidirectional recording/stimulation sites on a total active area of 2.28 mm x 1.14 mm, and a fully digital interface. Full frame recording rate is 2.4 kHz. Selection of a subset of sites is possible and translates into higher bandwidth per site accordingly. Electrical and biological results are presented which reveal proper functionality.
Figure 6. Two-way interfacing of a snail neuron. Top: Portion of the 32k pixel array with a cultured nerve cell from the pond snail, capacitively stimulated by a subarray of pixels (through off-chip generated signals in this case). Response recorded with a single pixel as indicated. The cell is not interfaced at the cell body, but at an extended neurite that is not visible in the micrograph. Bottom: Intracellular voltage (red) measured with an impaled micropipette, and extracellular voltage (black) measured with one pixel. Series of 35 biphasic voltage ramps (see insert) applied within 70 ms to the subset of pixels.
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