Tomography Toolbox

tom_acquire_tracking

correct for the  induced shifts due to the mechanically imprefectness of the stage and center the
object of interest.

Syntax

[break_fl,Shifts_diff,Tiltseries,Tracking]=tom_acquire_tracking(Tiltseries,Tracking,Acq_Area_Bin_fact,COMS)

Input:

Tiltseries	:state (structure), which includes the Values to set up tiltseries (Tiltangles, Filename ...)
Tracking	:state (structure), to set microscope in Tracking mode
Acq_Area_Bin_fact	:Acquisition.CCD.Area./Acquisition.CCD.Binning used to calculate  coordinates of a clicked markerpoint in the acquired image
COMS	:structure including the interface to the microcoscope (ActiveX Server)
to check the organisation of the structures see tom_make_acquisition_structure and tom_make_all_coms
Output
break_fl	:flag to stop the tiltseries
Shifts_diff:	:imagebeamshifts that are applied to the microscope
Tiltseries	:state (structure), which includes the values to setup the tiltseries (Tiltangles, Filename ...)
Tracking	:state (structure), to set microscope in Tracking mode

Description

tom_acquire_tracking corrects the movement of the stage (with
image/beamshift or stage) by centering/clicking a detail or by
crosscorrelation. If you use center detail the Coordinates of the clicked
marker will be written in the EM-header and can be used for further alignment steps.

Examples

See Also

tom_make_acquisition_structure, tom_make_all_Coms, tom_acquire_tracking,
tom_acquire_focus, tom_center_slit, tom_acquire_acquisition

tom_acquire_image

tom_calc_exptime