1. Acquire

Acquire a tilt series with tom_tilt_series.

Result: tilt series

 

 

2. Sort

Sort the acquired tilt series according to their tilt angle with tom_processdirgui

Result: sorted tilt series

 

 

3. Align

Align the sorted tilt series with tom_setmark

Result: markerfile.em

 

 

4. Reconstruct

Make a reconstruction with tom_Rec3dGUI. Just load the markerfile and the projections. The file includes the alignment parameters and the path of the tilt series.

Result: volume in .em format

 

 

5. Visualize

Visualize the volume with tom_volxyz

 

 

 

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© 2012, Max Planck Institute of Biochemistry, Martinsried