Contact

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Brenda Schulman, Ph.D.
Director
Phone:+49 89 8578

MPI of Biochemistry, Am Klopferspitz 18, 82152 Martinsried

Molecular Machines and Signaling

Publications

2017


Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase.
Scott DC, Hammill JT, Min J, Rhee DY, Connelly M, Sviderskiy VO, Bhasin D, Chen Y, Ong SS, Chai SC, Goktug AN, Huang G, Monda JK, Low J, Kim HS, Paulo JA, Cannon JR, Shelat AA, Chen T, Kelsall IR, Alpi AF, Pagala V, Wang X, Peng J, Singh B, Harper JW, Schulman BA*, Guy RK*.
Nat Chem Biol. 2017 Jun 5. doi: 10.1038/nchembio.2386.

Structural Studies of HHARI/UbcH7Ub Reveal Unique E2Ub Conformational Restriction by RBR RING1.
Dove KK, Olszewski JL, Martino L, Duda DM, Wu XS, Miller DJ, Reiter KH, Rittinger K, Schulman BA*, Klevit RE*.
Structure. 2017 Jun 6;25(6):890-900.e5. doi: 10.1016/j.str.2017.04.013.
• Preview by Dunkerley & Shaw: RBR Ubiquitin Transfer: Not Simply an "Open" and "Closed" Case?

Insights into links between autophagy and the ubiquitin system from the structure of LC3B bound to the LIR motif from the E3 ligase NEDD4.
Qiu Y, Zheng Y, Wu KP, Schulman BA.
Protein Sci. 2017 Aug;26(8):1674-1680. doi: 10.1002/pro.3186.

Production of Human ATG Proteins for Lipidation Assays.
Zheng Y, Qiu Y, Gunderson JE, Schulman BA.
Methods Enzymol. 2017;587:97-113. doi: 10.1016/bs.mie.2016.09.055.

Crystallographic Characterization of ATG Proteins and Their Interacting Partners.
Qiu Y, Zheng Y, Taherbhoy AM, Kaiser SE, Schulman BA.
Methods Enzymol. 2017;587:227-246. doi: 10.1016/bs.mie.2016.09.058.

2016


Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation.
Scott DC, Rhee DY, Duda DM, Kelsall IR, Olszewski JL, Paulo JA, de Jong A, Ovaa H, Alpi AF, Harper JW*, Schulman BA*.
Cell. 2016 Aug 25;166(5):1198-1214.e24. doi: 10.1016/j.cell.2016.07.027.
• Preview by Kleiger & Deshaies: Tag Team Ubiquitin Ligases.

Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation.
Yamaguchi M, VanderLinden R, Weissmann F, Qiao R, Dube P, Brown NG, Haselbach D, Zhang W, Sidhu SS, Peters JM*, Stark H*, Schulman BA*.
Mol Cell. 2016 Aug 18;63(4):593-607. doi: 10.1016/j.molcel.2016.07.003.
• News and Views by Morgan: Cell division: Mitotic regulation comes into focus.

Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.
Brown NG, VanderLinden R, Watson ER, Weissmann F, Ordureau A, Wu KP, Zhang W, Yu S, Mercredi PY, Harrison JS, Davidson IF, Qiao R, Lu Y, Dube P, Brunner MR, Grace CRR, Miller DJ, Haselbach D, Jarvis MA, Yamaguchi M, Yanishevski D, Petzold G, Sidhu SS, Kuhlman B, Kirschner MW, Harper JW, Peters JM*, Stark H*, Schulman BA*.
Cell. 2016 Jun 2;165(6):1440-1453. doi: 10.1016/j.cell.2016.05.037.

A cascading activity-based probe sequentially targets E1-E2-E3 ubiquitin enzymes.
Mulder MP, Witting K, Berlin I, Pruneda JN, Wu KP, Chang JG, Merkx R, Bialas J, Groettrup M, Vertegaal AC, Schulman BA, Komander D, Neefjes J, El Oualid F, Ovaa H.
Nat Chem Biol. 2016 Jul;12(7):523-30. doi: 10.1038/nchembio.2084.

Mechanism of APC/CCDC20 activation by mitotic phosphorylation.
Qiao R, Weissmann F, Yamaguchi M, Brown NG, VanderLinden R, Imre R, Jarvis MA, Brunner MR, Davidson IF, Litos G, Haselbach D, Mechtler K, Stark H*, Schulman BA*, Peters JM*.
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2570-8. doi: 10.1073/pnas.1604929113.

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes.
Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown NG, Lampert F, Westermann S, Stark H, Schulman BA*, Peters JM*.
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 10.1073/pnas.1604935113.

A role of autophagy in spinocerebellar ataxia-Rare exception or general principle?
Burmeister M, Lee JH, Schulman BA, Yapici Z, Tolun A, Juhasz G, Li JZ, Klionsky DJ.
Autophagy. 2016 Jul 2;12(7):1208-9. doi: 10.1080/15548627.2016.1170266.

System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes.
Zhang W, Wu KP, Sartori MA, Kamadurai HB, Ordureau A, Jiang C, Mercredi PY, Murchie R, Hu J, Persaud A, Mukherjee M, Li N, Doye A, Walker JR, Sheng Y, Hao Z, Li Y, Brown KR, Lemichez E, Chen J, Tong Y, Harper JW, Moffat J, Rotin D, Schulman BA*, Sidhu SS*.
Mol Cell. 2016 Apr 7;62(1):121-36. doi: 10.1016/j.molcel.2016.02.005.
• Preview by Canadeo & Huibregtse: A Billion Ubiquitin Variants to Probe and Modulate the UPS.

Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay.
Kim M, Sandford E, Gatica D, Qiu Y, Liu X, Zheng Y, Schulman BA, Xu J, Semple I, Ro SH, Kim B, Mavioglu RN, Tolun A, Jipa A, Takats S, Karpati M, Li JZ, Yapici Z, Juhasz G, Lee JH, Klionsky DJ, Burmeister M.
Elife. 2016 Jan 26;5. pii: e12245. doi: 10.7554/eLife.12245.

Multiple Weak Linear Motifs Enhance Recruitment and Processivity in SPOP-Mediated Substrate Ubiquitination.
Pierce WK, Grace CR, Lee J, Nourse A, Marzahn MR, Watson ER, High AA, Peng J, Schulman BA, Mittag T.
J Mol Biol. 2016 Mar 27;428(6):1256-71. doi: 10.1016/j.jmb.2015.10.002.

Measuring APC/C-Dependent Ubiquitylation In Vitro.
Jarvis MA, Brown NG, Watson ER, VanderLinden R, Schulman BA*, Peters JM*.
Methods Mol Biol. 2016;1342:287-303. doi: 10.1007/978-1-4939-2957-3_18.

2015


Itch WW Domains Inhibit Its E3 Ubiquitin Ligase Activity by Blocking E2-E3 Ligase Trans-thiolation.

Riling C, Kamadurai H, Kumar S, O'Leary CE, Wu KP, Manion EE, Ying M, Schulman BA, Oliver PM.
J Biol Chem. 2015 Sep 25;290(39):23875-87. doi: 10.1074/jbc.M115.649269.

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space.
Chari A, Haselbach D, Kirves JM, Ohmer J, Paknia E, Fischer N, Ganichkin O, Möller V, Frye JJ, Petzold G, Jarvis M, Tietzel M, Grimm C, Peters JM, Schulman BA, Tittmann K, Markl J, Fischer U, Stark H.
Nat Methods. 2015 Sep;12(9):859-65. doi: 10.1038/nmeth.3493

Defining roles of PARKIN and ubiquitin phosphorylation by PINK1 in mitochondrial quality control using a ubiquitin replacement strategy.
Ordureau A, Heo JM, Duda DM, Paulo JA, Olszewski JL, Yanishevski D, Rinehart J, Schulman BA*, Harper JW*.
Proc Natl Acad Sci U S A. 2015 May 26;112(21):6637-42. doi: 10.1073/pnas.1506593112.

PAX5 is a tumor suppressor in mouse mutagenesis models of acute lymphoblastic leukemia.
Dang J, Wei L, de Ridder J, Su X, Rust AG, Roberts KG, Payne-Turner D, Cheng J, Ma J, Qu C, Wu G, Song G, Huether RG, Schulman B, Janke L, Zhang J, Downing JR, van der Weyden L, Adams DJ, Mullighan CG.
Blood. 2015 Jun 4;125(23):3609-17. doi: 10.1182/blood-2015-02-626127.

RING E3 mechanism for ubiquitin ligation to a disordered substrate visualized for human anaphase-promoting complex.
Brown NG, VanderLinden R, Watson ER, Qiao R, Grace CR, Yamaguchi M, Weissmann F, Frye JJ, Dube P, Ei Cho S, Actis ML, Rodrigues P, Fujii N, Peters JM*, Stark H*, Schulman BA*.
Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):5272-9. doi: 10.1073/pnas.1504161112.

Protein neddylation: beyond cullin-RING ligases.
Enchev RI, Schulman BA, Peter M.
Nat Rev Mol Cell Biol. 2015 Jan;16(1):30-44. doi: 10.1038/nrm3919.

Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome.
Yamaguchi M, Yu S, Qiao R, Weissmann F, Miller DJ, VanderLinden R, Brown NG, Frye JJ, Peters JM, Schulman BA.
J Mol Biol. 2015 Apr 24;427(8):1748-64. doi: 10.1016/j.jmb.2014.11.020.

2014


Mechanism of polyubiquitination by human anaphase-promoting complex: RING repurposing for ubiquitin chain assembly.
Brown NG, Watson ER, Weissmann F, Jarvis MA, VanderLinden R, Grace CR, Frye JJ, Qiao R, Dube P, Petzold G, Cho SE, Alsharif O, Bao J, Davidson IF, Zheng JJ, Nourse A, Kurinov I, Peters JM*, Stark H*, Schulman BA*.
Mol Cell. 2014 Oct 23;56(2):246-60. doi: 10.1016/j.molcel.2014.09.009.
• Preview by Iwai & Tanaka: Ubiquitin chain elongation: an intriguing strategy.

Quantitative proteomics reveal a feedforward mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis.
Ordureau A, Sarraf SA, Duda DM, Heo JM, Jedrychowski MP, Sviderskiy VO, Olszewski JL, Koerber JT, Xie T, Beausoleil SA, Wells JA, Gygi SP, Schulman BA, Harper JW.
Mol Cell. 2014 Nov 6;56(3):360-75. doi: 10.1016/j.molcel.2014.09.007.
• Preview by Stolz & Dikic: PINK1-PARKIN interplay: down to ubiquitin phosphorylation.

Structure of a RING E3 trapped in action reveals ligation mechanism for the ubiquitin-like protein NEDD8.
Scott DC, Sviderskiy VO, Monda JK, Lydeard JR, Cho SE, Harper JW, Schulman BA.
Cell. 2014 Jun 19;157(7):1671-84. doi: 10.1016/j.cell.2014.04.037.

The instability of the BTB-KELCH protein Gigaxonin causes Giant Axonal Neuropathy and constitutes a new penetrant and specific diagnostic test.
Boizot A, Talmat-Amar Y, Morrogh D, Kuntz NL, Halbert C, Chabrol B, Houlden H, Stojkovic T, Schulman BA, Rautenstrauss B, Bomont P.
Acta Neuropathol Commun. 2014 Apr 24;2:47. doi: 10.1186/2051-5960-2-47.

Atomistic autophagy: the structures of cellular self-digestion.
Hurley JH, Schulman BA.
Cell. 2014 Apr 10;157(2):300-311. doi: 10.1016/j.cell.2014.01.070.

Dynamic regulation of macroautophagy by distinctive ubiquitin-like proteins.
Klionsky DJ, Schulman BA.
Nat Struct Mol Biol. 2014 Apr;21(4):336-45. doi: 10.1038/nsmb.2787.

SPOP promotes tumorigenesis by acting as a key regulatory hub in kidney cancer.
Li G, Ci W, Karmakar S, Chen K, Dhar R, Fan Z, Guo Z, Zhang J, Ke Y, Wang L, Zhuang M, Hu S, Li X, Zhou L, Li X, Calabrese MF, Watson ER, Prasad SM, Rinker-Schaeffer C, Eggener SE, Stricker T, Tian Y, Schulman BA, Liu J, White KP.
Cancer Cell. 2014 Apr 14;25(4):455-68. doi: 10.1016/j.ccr.2014.02.007.

2013


Building and remodelling Cullin-RING E3 ubiquitin ligases.
Lydeard JR, Schulman BA, Harper JW.
EMBO Rep. 2013 Dec;14(12):1050-61. doi: 10.1038/embor.2013.173.

Binding to E1 and E3 is mutually exclusive for the human autophagy E2 Atg3.
Qiu Y, Hofmann K, Coats JE, Schulman BA, Kaiser SE.
Protein Sci. 2013 Dec;22(12):1691-7. doi: 10.1002/pro.2381.

TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes.
Kelsall IR, Duda DM, Olszewski JL, Hofmann K, Knebel A, Langevin F, Wood N, Wightman M, Schulman BA, Alpi AF.
EMBO J. 2013 Oct 30;32(21):2848-60. doi: 10.1038/emboj.2013.209.

Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3.
Kamadurai HB, Qiu Y, Deng A, Harrison JS, Macdonald C, Actis M, Rodrigues P, Miller DJ, Souphron J, Lewis SM, Kurinov I, Fujii N, Hammel M, Piper R, Kuhlman B, Schulman BA.
Elife. 2013 Aug 8;2:e00828. doi: 10.7554/eLife.00828.
• Insight by Meyer & Rape: Caught in the act.

Electron microscopy structure of human APC/C(CDH1)-EMI1 reveals multimodal mechanism of E3 ligase shutdown.
Frye JJ, Brown NG, Petzold G, Watson ER, Grace CR, Nourse A, Jarvis MA, Kriwacki RW, Peters JM*, Stark H*, Schulman BA*.
Nat Struct Mol Biol. 2013 Jul;20(7):827-35. doi: 10.1038/nsmb.2593.
• News & Views by Yamano: EMI1, a three-in-one ubiquitylation inhibitor.
• Leading Edge: Intrinsically disordered
proteins, https://doi.org/10.1016/j.cell.2013.07.026

Structure of HHARI, a RING-IBR-RING ubiquitin ligase: autoinhibition of an Ariadne-family E3 and insights into ligation mechanism.
Duda DM, Olszewski JL, Schuermann JP, Kurinov I, Miller DJ, Nourse A, Alpi AF, Schulman BA.
Structure. 2013 Jun 4;21(6):1030-41. doi: 10.1016/j.str.2013.04.019.
• Preview by Davies & Das: HHARI is one HECT of a RING.
• Have you seen? by Byrd & Weissman:  Compact Parkin only: insights into the structure of an autoinhibited ubiquitin ligase.

Structures of Atg7-Atg3 and Atg7-Atg10 reveal noncanonical mechanisms of E2 recruitment by the autophagy E1.
Kaiser SE, Qiu Y, Coats JE, Mao K, Klionsky DJ, Schulman BA.
Autophagy. 2013 May;9(5):778-80. doi: 10.4161/auto.23644.

SCFFbxw5 mediates transient degradation of actin remodeller Eps8 to allow proper mitotic progression.
Werner A, Disanza A, Reifenberger N, Habeck G, Becker J, Calabrese M, Urlaub H, Lorenz H, Schulman B, Scita G, Melchior F.
Nat Cell Biol. 2013 Feb;15(2):179-88. doi: 10.1038/ncb2661.

Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes.
Monda JK, Scott DC, Miller DJ, Lydeard J, King D, Harper JW, Bennett EJ, Schulman BA.
Structure. 2013 Jan 8;21(1):42-53. doi: 10.1016/j.str.2012.10.013.

2012


­Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures.
Kaiser SE, Mao K, Taherbhoy AM, Yu S, Olszewski JL, Duda DM, Kurinov I, Deng A, Fenn TD, Klionsky DJ, Schulman BA.
Nat Struct Mol Biol. 2012 Dec;19(12):1242-9. doi: 10.1038/nsmb.2415.
• Research Highlights by Donner: E2 loader, doi:10.1038/nchembio.1145

APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.
Uzunova K, Dye BT, Schutz H, Ladurner R, Petzold G, Toyoda Y, Jarvis MA, Brown NG, Poser I, Novatchkova M, Mechtler K, Hyman AA, Stark H, Schulman BA, Peters JM.
Nat Struct Mol Biol. 2012 Nov;19(11):1116-23. doi: 10.1038/nsmb.2412.
• News and Views by Musacchio & Ciliberto: The spindle-assembly checkpoint and the beauty of self-destruction.

Structural basis for a reciprocal regulation between SCF and CSN.
Enchev RI, Scott DC, da Fonseca PC, Schreiber A, Monda JK, Schulman BA*, Peter M*, Morris EP*.
Cell Rep. 2012 Sep 27;2(3):616-27. doi: 10.1016/j.celrep.2012.08.019.

Structural biology: A protein engagement RING.
Lima CD, Schulman BA.
Nature. 2012 Sep 6;489(7414):43-4. doi: 10.1038/489043a.

Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface.
Duda DM, Olszewski JL, Tron AE, Hammel M, Lambert LJ, Waddell MB, Mittag T, DeCaprio JA, Schulman BA.
Mol Cell. 2012 Aug 10;47(3):371-82. doi: 10.1016/j.molcel.2012.05.044.
• Preview by Hristova, Stringer & Weissman: Cullin RING ligases: glommed by glomulin.

Ubiquitin-like modifiers.
Taherbhoy AM, Schulman BA, Kaiser SE.
Essays Biochem. 2012;52:51-63. doi: 10.1042/bse0520051.

The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7.
Tron AE, Arai T, Duda DM, Kuwabara H, Olszewski JL, Fujiwara Y, Bahamon BN, Signoretti S, Schulman BA, DeCaprio JA.
Mol Cell. 2012 Apr 13;46(1):67-78. doi: 10.1016/j.molcel.2012.02.005.

Viral E3 ubiquitin ligase-mediated degradation of a cellular E3: viral mimicry of a cellular phosphorylation mark targets the RNF8 FHA domain.
Chaurushiya MS, Lilley CE, Aslanian A, Meisenhelder J, Scott DC, Landry S, Ticau S, Boutell C, Yates JR 3rd, Schulman BA, Hunter T, Weitzman MD.
Mol Cell. 2012 Apr 13;46(1):79-90. doi: 10.1016/j.molcel.2012.02.004.

Trans mechanism for ubiquitin-like protein transfer in autophagy.
Taherbhoy AM, Kaiser SE, Schulman BA.
Cell Cycle. 2012 Feb 15;11(4):635-6. doi: 10.4161/cc.11.4.19277.

2011


­Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway.
Taherbhoy AM, Tait SW, Kaiser SE, Williams AH, Deng A, Nourse A, Hammel M, Kurinov I, Rock CO, Green DR, Schulman BA.
Mol Cell. 2011 Nov 4;44(3):451-61. doi: 10.1016/j.molcel.2011.08.034.

Twists and turns in ubiquitin-like protein conjugation cascades.
Schulman BA.
Protein Sci. 2011 Dec;20(12):1941-54. doi: 10.1002/pro.750.

N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex.
Scott DC, Monda JK, Bennett EJ, Harper JW, Schulman BA.
Science. 2011 Nov 4;334(6056):674-8. doi: 10.1126/science.1209307.
• Research Highlights by Mason: Acetylating for avidity, doi:10.1038/nsmb.2181

A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases.
Calabrese MF, Scott DC, Duda DM, Grace CR, Kurinov I, Kriwacki RW, Schulman BA.
Nat Struct Mol Biol. 2011 Jul 17;18(8):947-9. doi: 10.1038/nsmb.2086.
• News and Views by Rahighi & Dikic: Conformational flexibility and rotation of the RING domain in activation of cullin-RING ligases.

Structural regulation of cullin-RING ubiquitin ligase complexes.
Duda DM, Scott DC, Calabrese MF, Zimmerman ES, Zheng N*, Schulman BA*.
Curr Opin Struct Biol. 2011 Apr;21(2):257-64. doi: 10.1016/j.sbi.2011.01.003.

IDH1 and IDH2 mutations in pediatric acute leukemia.
Andersson AK, Miller DW, Lynch JA, Lemoff AS, Cai Z, Pounds SB, Radtke I, Yan B, Schuetz JD, Rubnitz JE, Ribeiro RC, Raimondi SC, Zhang J, Mullighan CG, Shurtleff SA, Schulman BA, DowningJR.
Leukemia. 2011 Oct;25(10):1570-7. doi: 10.1038/leu.2011.133.

2010


­Crystal structure of UBA2(ufd)-Ubc9: insights into E1-E2 interactions in Sumo pathways.
Wang J, Taherbhoy AM, Hunt HW, Seyedin SN, Miller DW, Miller DJ, Huang DT, Schulman BA.
PLoS One. 2010 Dec 30;5(12):e15805. doi: 10.1371/journal.pone.0015805.

Pathogenic bacteria target NEDD8-conjugated cullins to hijack host-cell signaling pathways.
Jubelin G, Taieb F, Duda DM, Hsu Y, Samba-Louaka A, Nobe R, Penary M, Watrin C, Nougayrède JP, Schulman BA, Stebbins CE, Oswald E.
PLoS Pathog. 2010 Sep 30;6(9):e1001128. doi: 10.1371/journal.ppat.1001128.

Structural assembly of cullin-RING ubiquitin ligase complexes.
Zimmerman ES, Schulman BA, Zheng N.
Curr Opin Struct Biol. 2010 Dec;20(6):714-21. doi: 10.1016/j.sbi.2010.08.010.

A dual E3 mechanism for Rub1 ligation to Cdc53.
Scott DC, Monda JK, Grace CR, Duda DM, Kriwacki RW, Kurz T, Schulman BA.
Mol Cell. 2010 Sep 10;39(5):784-96. doi: 10.1016/j.molcel.2010.08.030.

Structural biology: Transformative encounters.
Schulman BA, Haas AL.
Nature. 2010 Feb 18;463(7283):889-90. doi: 10.1038/463889a.

Identification and characterization of the first small molecule inhibitor of MDMX.
Reed D, Shen Y, Shelat AA, Arnold LA, Ferreira AM, Zhu F, Mills N, Smithson DC, Regni CA, Bashford D, Cicero SA, Schulman BA, Jochemsen AG, Guy RK, Dyer MA.
J Biol Chem. 2010 Apr 2;285(14):10786-96. doi: 10.1074/jbc.M109.056747.

2009


Insights into ubiquitin transfer cascades from a structure of a UbcH5B~ubiquitin-HECT(NEDD4L) complex.
Kamadurai HB, Souphron J, Scott DC, Duda DM, Miller DJ, Stringer D, Piper RC, Schulman BA.
Mol Cell. 2009 Dec 25;36(6):1095-102. doi: 10.1016/j.molcel.2009.11.010.

Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae.
Siergiejuk E, Scott DC, Schulman BA, Hofmann K, Kurz T, Peter M.
EMBO J. 2009 Dec 16;28(24):3845-56. doi: 10.1038/emboj.2009.354.

Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA.
Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.

Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure.
Wang J, Dye BT, Rajashankar KR, Kurinov I, Schulman BA.
Nat Struct Mol Biol. 2009 Sep;16(9):987-9. doi: 10.1038/nsmb.1645.

(G2)BRinging an E2 to E3.
Wang J, Schulman BA.
Structure. 2009 Jul 15;17(7):916-7. doi: 10.1016/j.str.2009.06.005.

How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic.
Regni CA, Roush RF, Miller DJ, Nourse A, Walsh CT, Schulman BA.
EMBO J. 2009 Jul 8;28(13):1953-64. doi: 10.1038/emboj.2009.146.

JAK mutations in high-risk childhood acute lymphoblastic leukemia.
Mullighan CG, Zhang J, Harvey RC, Collins-Underwood JR, Schulman BA, Phillips LA, Tasian SK, Loh ML, Su X, Liu W, Devidas M, Atlas SR, Chen IM, Clifford RJ, Gerhard DS, Carroll WL, Reaman GH, Smith M, Downing JR, Hunger SP, Willman CL.
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9414-8. doi: 10.1073/pnas.0811761106.

Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways.
Schulman BA, Harper JW.
Nat Rev Mol Cell Biol. 2009 May;10(5):319-31. doi: 10.1038/nrm2673.

E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF*, Schulman BA*.
Mol Cell. 2009 Feb 27;33(4):483-95. doi: 10.1016/j.molcel.2009.01.011.
• Preview by Pichler: A second E2 for nedd8ylation expands substrate selection.

Deletion of IKZF1 and prognosis in acute lymphoblastic leukemia.
Mullighan CG, Su X, Zhang J, Radtke I, Phillips LA, Miller CB, Ma J, Liu W, Cheng C, Schulman BA, Harvey RC, Chen IM, Clifford RJ, Carroll WL, Reaman G, Bowman WP, Devidas M, Gerhard DS, Yang W, Relling MV, Shurtleff SA, Campana D, Borowitz MJ, Pui CH, Smith M, Hunger SP, Willman CL, Downing JR; Children's Oncology Group.
N Engl J Med. 2009 Jan 29;360(5):470-80. doi: 10.1056/NEJMoa0808253.

2008


­Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation.
Duda DM, Borg LA, Scott DC, Hunt HW, Hammel M, Schulman BA.
Cell. 2008 Sep 19;134(6):995-1006. doi: 10.1016/j.cell.2008.07.022.
• Preview by Saifee & Zheng: A ubiquitin-like protein unleashes ubiquitin ligases.

Structural dissection of a gating mechanism preventing misactivation of ubiquitin by NEDD8's E1.
Souphron J, Waddell MB, Paydar A, Tokgöz-Gromley Z, Roussel MF, Schulman BA.
Biochemistry. 2008 Aug 26;47(34):8961-9. doi: 10.1021/bi800604c.

Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2.
Huang DT, Zhuang M, Ayrault O, Schulman BA.
Nat Struct Mol Biol. 2008 Mar;15(3):280-7. doi: 10.1038/nsmb.1387.

2007


­Structural mechanisms underlying posttranslational modification by ubiquitin-like proteins.
Dye BT, Schulman BA.
Annu Rev Biophys Biomol Struct. 2007;36:131-50.

Structure of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p: conservation of a non-covalent ubiquitin-like protein-E2 complex as a platform for selective interactions within a SUMO pathway.
Duda DM, van Waardenburg RC, Borg LA, McGarity S, Nourse A, Waddell MB, Bjornsti MA, Schulman BA.
J Mol Biol. 2007 Jun 8;369(3):619-30.

Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity.
Huang DT, Hunt HW, Zhuang M, Ohi MD, Holton JM, Schulman BA.
Nature. 2007 Jan 25;445(7126):394-8.
• News and Views by Trempe & Endicott: Structural biology: pass the protein.
• Preview by Haas:  Structural insights into early events in the conjugation of ubiquitin and ubiquitin-like proteins.

2006


­Breaking up with a kinky SUMO.
Huang DT, Schulman BA.
Nat Struct Mol Biol. 2006 Dec;13(12):1045-7.

Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress.
van Waardenburg RC, Duda DM, Lancaster CS, Schulman BA, Bjornsti MA.
Mol Cell Biol. 2006 Jul;26(13):4958-69.

Structural complexity in ubiquitin recognition.
Harper JW, Schulman BA.
Cell. 2006 Mar 24;124(6):1133-6.

A calcium-regulated MEF2 sumoylation switch controls postsynaptic differentiation.
Shalizi A, Gaudillière B, Yuan Z, Stegmüller J, Shirogane T, Ge Q, Tan Y, Schulman B, Harper JW, Bonni A.
Science. 2006 Feb 17;311(5763):1012-7.

2005


­Protein ubiquitination: CHIPping away the symmetry.
Schulman BA, Chen ZJ.
Mol Cell. 2005 Dec 9;20(5):653-5.

High-level expression and purification of recombinant SCF ubiquitin ligases.
Li T, Pavletich NP*, Schulman BA*, Zheng N*.
Methods Enzymol. 2005;398:125-42.

Expression, purification, and characterization of the E1 for human NEDD8, the heterodimeric APPBP1-UBA3 complex.
Huang DT, Schulman BA.
Methods Enzymol. 2005;398:9-20.

Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase.
Hao B, Zheng N, Schulman BA, Wu G, Miller JJ, Pagano M, Pavletich NP.
Mol Cell. 2005 Oct 7;20(1):9-19.

E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer.
Eletr ZM, Huang DT, Duda DM, Schulman BA, Kuhlman B.
Nat Struct Mol Biol. 2005 Oct;12(10):933-4.

Tag-team SUMO wrestling.
Duda DM, Schulman BA.
Mol Cell. 2005 Jun 10;18(6):612-4.

Structural analysis of Escherichia coli ThiF.
Duda DM, Walden H, Sfondouris J, Schulman BA.
J Mol Biol. 2005 Jun 17;349(4):774-86.

Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1.
Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA.
Mol Cell. 2005 Feb 4;17(3):341-50.
• Preview by VanDemark & Hill: E1 on the move.

2004


­A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8.
Huang DT, Miller DW, Mathew R, Cassell R, Holton JM, Roussel MF, Schulman BA.
Nat Struct Mol Biol. 2004 Oct;11(10):927-35.
• Preview by VanDemark & Hill: Grabbing E2 by the tail.

Structural and dynamic independence of isopeptide-linked RanGAP1 and SUMO-1.
Macauley MS, Errington WJ, Okon M, Schärpf M, Mackereth CD, Schulman BA, McIntosh LP.
J Biol Chem. 2004 Nov 19;279(47):49131-7.

The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein.
Zhao C, Beaudenon SL, Kelley ML, Waddell MB, Yuan W, Schulman BA, Huibregtse JM, Krug RM.
Proc Natl Acad Sci U S A. 2004 May 18;101(20):7578-82.

Local and global cooperativity in the human alpha-lactalbumin molten globule.
Quezada CM, Schulman BA, Froggatt JJ, Dobson CM, Redfield C.
J Mol Biol. 2004 Apr 16;338(1):149-58.

Ubiquitin-like protein activation.
Huang DT, Walden H, Duda D, Schulman BA.
Oncogene. 2004 Mar 15;23(11):1958-71.

2003


The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1.
Walden H, Podgorski MS, Huang DT, Miller DW, Howard RJ, Minor DL Jr, Holton JM, Schulman BA.
Mol Cell. 2003 Dec;12(6):1427-37.

Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase.
Wu G, Xu G, Schulman BA, Jeffrey PD, Harper JW, Pavletich NP.
Mol Cell. 2003 Jun;11(6):1445-56.

Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA.
Nature. 2003 Mar 20;422(6929):330-4.
• News and Views by VanDemark & Hill: Two-stepping with E1.

Parkin is a component of an SCF-like ubiquitin ligase complex and protects postmitotic neurons from kainate excitotoxicity.
Staropoli JF, McDermott C, Martinat C, Schulman B, Demireva E, Abeliovich A.
Neuron. 2003 Mar 6;37(5):735-49.

2002


­Identification of a multifunctional binding site on Ubc9p required for Smt3p conjugation.
Bencsath KP, Podgorski MS, Pagala VR, Slaughter CA, Schulman BA.
J Biol Chem. 2002 Dec 6;277(49):47938-45.

The SOCS box: a tale of destruction and degradation.
Kile BT, Schulman BA, Alexander WS, Nicola NA, Martin HM, Hilton DJ.
Trends Biochem Sci. 2002 May;27(5):235-41.

Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex.
Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P, Chu C, Koepp DM, Elledge SJ, Pagano M, Conaway RC, Conaway JW, Harper JW, Pavletich NP.
Nature. 2002 Apr 18;416(6882):703-9.

2001 and earlier
­


Probing subtle differences in the hydrogen exchange behavior of variants of the human alpha-lactalbumin molten globule using mass spectrometry.
Last AM, Schulman BA, Robinson CV, Redfield C.
J Mol Biol. 2001 Aug 24;311(4):909-19.

A splice variant of Skp2 is retained in the cytoplasm and fails to direct cyclin D1 ubiquitination in the uterine cancer cell line SK-UT.
Ganiatsas S, Dow R, Thompson A, Schulman B, Germain D.
Oncogene. 2001 Jun 21;20(28):3641-50.

Role of the F-box protein Skp2 in lymphomagenesis.
Latres E, Chiarle R, Schulman BA, Pavletich NP, Pellicer A, Inghirami G, Pagano M.
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2515-20.

Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex.
Schulman BA, Carrano AC, Jeffrey PD, Bowen Z, Kinnucan ER, Finnin MS, Elledge SJ, Harper JW, Pagano M, Pavletich NP.
Nature. 2000 Nov 16;408(6810):381-6.

Alpha-lactalbumin forms a compact molten globule in the absence of disulfide bonds.
Redfield C, Schulman BA, Milhollen MA, Kim PS, Dobson CM.
Nat Struct Biol. 1999 Oct;6(10):948-52.

Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A.
Schulman BA, Lindstrom DL, Harlow E.
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10453-8.

A residue-specific NMR view of the non-cooperative unfolding of a molten globule.
Schulman BA, Kim PS, Dobson CM, Redfield C.
Nat Struct Biol. 1997 Aug;4(8):630-4.

Proline scanning mutagenesis of a molten globule reveals non-cooperative formation of a protein's overall topology.
Schulman BA, Kim PS.
Nat Struct Biol. 1996 Aug;3(8):682-7.

Disulfide determinants of calcium-induced packing in alpha-lactalbumin.
Wu LC, Schulman BA, Peng ZY, Kim PS.
Biochemistry. 1996 Jan 23;35(3):859-63.

Different subdomains are most protected from hydrogen exchange in the molten globule and native states of human alpha-lactalbumin.
Schulman BA, Redfield C, Peng ZY, Dobson CM, Kim PS.
J Mol Biol. 1995 Nov 10;253(5):651-7.

Does the molten globule have a native-like tertiary fold?
Peng ZY, Wu LC, Schulman BA, Kim PS.
Philos Trans R Soc Lond B Biol Sci. 1995 Apr 29;348(1323):43-7.

Hydrogen exchange in BPTI variants that do not share a common disulfide bond.
Schulman BA, Kim PS.
Protein Sci. 1994 Dec;3(12):2226-32.

Hydrogen-oxidizing electron transport components in the hyperthermophilic archaebacterium Pyrodictium brockii.
Pihl TD, Black LK, Schulman BA, Maier RJ.
J Bacteriol. 1992 Jan;174(1):137-43.

Hydrogen-sulfur autotrophy in the hyperthermophilic archaebacterium, Pyrodictium brockii.
Pihl TD, Schicho RN, Black LK, Schulman BA, Maier RJ, Kelly RM.
Biotechnol Genet Eng Rev. 1990;8:345-77.

 
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