Curriculum vitae

 

 

Eri Sakata, Ph. D.

email: sakata@biochem.mpg.de

tel.: +49-89-8578-2642

 

 

Academic Education
YEAR INSTITUTION DEGREE FIELD OF STUDY
2007 Nagoya City University Ph.D. Pharmaceutical Sciences
2004 Nagoya City University M.Sc. Pharmaceutical Sciences
2002 Nagoya City University B.Sc. Pharmaceutical Sciences

 

 

Research Experience

 

2013-present Project Group Leader,
Max-Planck Institute of Biochemistry, Martinsried, Germany
Department of Molecular Structural Biology

2011-2013

Postdoctoral fellow (Uehara Postdoctoral fellow)

Yale University, New Haven, U.S.A.
Department of Molecular Biophysics & Biochemistry
(Prof. Mark Hochstrasser)

2007-2011 Joint postdoctoral fellow (JSPS Postdoctoral fellow)
Max-Planck Institute of Biochemistry, Martinsried, Germany
Department of Molecular Structural Biology
(Prof. Wolfgang Baumeister)
and
Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Laboratory of Frontier Science
(Prof. Keiji Tanaka)

2004-2007 Graduate student (JSPS Ph.D fellow)
Nagoya City University, Nagoya, Japan
Department of Structural Biology and Biomolecular Engineering
(Prof. Koichi Kato)

2002-2004 Graduate student (Master of Pharmaceutical Sciences)
Nagoya City University, Nagoya, Japan
Department of Structural Biology and Biomolecular Engineering
(Prof. Koichi Kato)

 

Publication list

 

Pack CG, Yukii H, Toh-e A, Kudo T, Tsuchiya H, Kaiho A, Sakata E, Murata S, Yokosawa H, Sako Y, Baumeister W, Tanaka K, Saeki Y, Quantitative live-cell imaging reveals spatio-temporal dynamics and cytoplasmic assembly of the 26S proteasome. Nat Commun. 5:3396 (2014)

 

Bohn S, Sakata E, Beck F, Pathare GR, Schnitger J, Nágy I, Baumeister W, Förster F. Localization of the regulatory particle subunit Sem1 in the 26S proteasome. Biochem Biophys Res Commun. 435(2):250-354 (2013)

 

Fernández-Busnadiego R, Asano S, Oprisoreanu AM, Sakata E, Doengi M, Kochovski Z, Zürner M, Stein V, Schoch S, Baumeister W, Lucić V. Cryo-electron tomography reveals a critical role of RIM1a in synaptic vesicle tethering. J Cell Biol. 201(5):725-740, (2013)

 

Sugiyama M, Sahashi H, Kurimoto E, Takata S, Yagi H, Kanai K, Sakata E, Minami Y, Tanaka K, Kato K. Spatial arrangement and functional role of α subunits of proteasome activator PA28 in hetero-oligomeric form. Biochem Biophys Res Commun. 432(1):141-145, (2013)

 

Uekusa Y, Mimura S, Sasakawa H, Kurimoto E, Sakata E, Olivier S, Yagi H, Tokunaga F, Iwai K, Kato K. Backbone and side chain 1H, 13C, and 15N assignments of the ubiquitin-like domain of human HOIL-1L, an essential component of linear ubiquitin chain assembly complex. Biomol NMR Assign. 6(2):177-180, (2012)

 

Beck F, Unverdorben P, Bohn S, Schweitzer A, Pfeifer G, Sakata E, Nickell S, Plitzko JM, Villa E, Baumeister W, Förster F. Near-atomic resolution structural model of the yeast 26S proteasome. Proc Natl Acad Sci U S A.109(37):14870-14875 (2012)

 

Sakata, E., Bohn, S., Miharache,O., Kiss, P., Beck, F., Nickell S., Tanaka, K., Saeki, Y., Förster F., Baumeister, W. Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy. Proc. Natl. Acad. Sci. U. S. A., 109, 1479-1484, (2012)

 

Sakata, E. *, Stengel, F.*,Fukunaga, K., Zhou, M., Saeki, Y., Förster, F., Tanaka, K., Baumeister, W., and Robinson, C.V. The catalytic activity of Ubp6 enhances maturation of the proteasomal regulatory particle. Mol. Cell, 42, 637-649, (2011)

* Equal contribution

 

Uekusa Y, Mimura S, Sasakawa H, Kurimoto E, Sakata E, Olivier S, Yagi H, Tokunaga F, Iwai K, Kato K. Backbone and side chain (1)H, (13)C, and (15)N assignments of the ubiquitin-like domain of human HOIL-1L, an essential component of linear ubiquitin chain assembly complex. Biomol NMR Assign. (2011)

 

Bohn, S., Beck, F., Sakata, E., Walzthoeni, T., Beck, B., Aebersold R., Förster F., Baumeister, W., Nickell, S. Structure of the 26S proteasome from Schizosaccharomyces pombe at subnanometer resolution. Proc. Natl. Acad. Sci. U. S. A., 107, 20992-20997 (2010)

 

Förster, F.and Sakata, E. Proteasome structure and function. Encyclopedia of Biological Chemistry, 2nd Edition, (2010)

 

Satoh,T. *, Sakata, E. *, Yamamoto, S., Yamaguchi, Y., Sumiyoshi, A., Wakatsuki, S. and Kato, K. Crystal structure of cyclic Lys48-linked tetraubiquitin., Biochem. Biophys. Res. Commun., 400, 329-333 (2010)

* Equal contribution

 

Sugiyama, M., Kurimoto, E., Sahashi, H., Sakata, E., Morimoto, Y., Itoh, K., Mori, K., Fukunaga, T., Minami, Y. and Kato K. SANS investigation of assembly state of proteasome activator 28 and the 20S proteasome. J. Phys.: Conf. Ser., 247, 12020 (2010)

 

Sakata, E.*, Satoh, T.*, Yamamoto, S., Yamaguchi, Y., Yagi-Utsumi, M., Kurimoto, E., Tanaka, K., Wakatsuki, S., and Kato, K. Crystal structure of UbcH5b~ubiquitin intermediate: Insight into the formation of the self-assembled E2~Ub conjugates. Structure, 18, 138-147 (2010)

* Equal contribution

 

Sugiyama, M., Kurimoto, E., Morimoto, Y., Sahashi, H., Sakata, E., Hamada, K., Itoh, K., Mori, K.,  Fukunaga, T., Minami, Y., and Kato, K. Assembly State of Proteasome Activator 28 in an Aqueous Solution as Studied by Small-Angle Neutron Scattering. J. Phys. Soc. Jpn., 78, 124802-124808 (2009)

 

Sakata, E., Saeki, Y., and Tanaka, K. The proteasome’s crown for destruction. Mol. Cell, 34, 519-520 (2009)

 

Yashiroda H., Mizushima T., Okamoto K., Kameyama T., Hayashi H., Kishimoto T., Niwa S., Kasahara M., Kurimoto E., Sakata E., Takagi K., Suzuki A., Hirano Y., Murata S., Kato K., Yamane T. and Tanaka K. Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes. Nat. Struct. Mol. Biol., 15, 228-236 (2008)

 

Sasakawa, H., Sakata, E., Yamaguchi, Y., Masuda, M., Mori, T., Kurimoto, E., Iguchi, T., Hisanaga, S., Iwatsubo, T., Hasegawa, M. and Kato, K.Ultra-high field NMR studies of antibody binding and site-specific phosphorylation of α-synuclein. Biochem. Biophys. Res. Commun. 363, 795-799 (2007)

 

Sakata, E., Yamaguchi, Y., Miyauchi, Y., Iwai, K., Chiba, T., Saeki, Y., Matsuda, N., Tanaka, K. and Kato, K. Direct interactions between NEDD8 and ubiquitin E2 conjugating enzymes upregulate cullin-based E3 ligase activity. Nat. Struct. Mol. Biol., 14, 167-168 (2007)

 

Yamaguchi, Y., Hirao, T., Sakata, E., Kamiya, Y., Kurimoto, E., Yoshida, Y., Suzuki, T., Tanaka, K. and Kato, K. Fbs1 protects the malfolded glycoproteins from the attack of peptide: N-glycanase. Biochem. Biophys. Res. Commun., 362, 712-716 (2007)

 

Sasakawa H., Sakata E., Yamaguchi Y., Komatsu M., Tatsumi K., Kominami E., Tanaka K. and Kato K. Solution structure and dynamics of Ufm1, a ubiquitin-fold modifier 1. Biochem. Biophys. Res. Commun., 343, 21-26 (2006)

 

Sato S., Chiba T., Sakata E., Kato K., Mizuno Y., Hattori N. and Tanaka K. 14-3-3η is a novel regulator of parkin ubiquitin-ligase. EMBO J. 25, 211-221 (2006)

 

Sakata E., Yamaguchi Y., Kurimoto E., Kikuchi J., Yokoyama S., Yamada S., Kawahara H., Yokosawa H., Hattori N., Mizuno Y., Tanaka K., and Kato K.

Parkin binds the Rpn10 subunit of 26S proteasomes through its ubiquitin-like domain. EMBO rep., 4, 301-306 (2003)

 

 

Awards, Grants & Fellowships

 

2013-2016         Marie Curie Career Integration Grant (CIG)

2011-2012         Postdoctoral Research Fellowship (Uehara Memorial Foundation)

2009                  Poster Award (Tokyo Metropolitan Institute of Medical Sciences)

2007-2010         Postdoctoral Research Fellowship (Japan Society for the Promotion of Science)

2003-2007         Ph.D Research Fellowship (Japan Society for the Promotion of Science)

2003                  Poster Award (15th Naito conference on molecular biological approaches for intractable diseases, Japan)

 

 
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